Osteoclast stimulatory transmembrane protein and dendritic cell-specific transmembrane protein cooperatively modulate cell-cell fusion to form osteoclasts and foreign body giant cells

Hiroya Miyamoto, Takayuki Suzuki, Yoshiteru Miyauchi, Ryotaro Iwasaki, Tami Kobayashi, Yuiko Sato, Kana Miyamoto, Hiroko Hoshi, Kazuaki Hashimoto, Shigeyuki Yoshida, Wu Hao, Tomoaki Mori, Hiroya Kanagawa, Eri Katsuyama, Atsuhiro Fujie, Hideo Morioka, Morio Matsumoto, Kazuhiro Chiba, Motohiro Takeya, Yoshiaki ToyamaTakeshi Miyamoto

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Cell-cell fusion is a dynamic phenomenon promoting cytoskeletal reorganization and phenotypic changes. To characterize factors essential for fusion of macrophage lineage cells, we identified the multitransmembrane protein, osteoclast stimulatory transmembrane protein (OC-STAMP), and analyzed its function. OC-STAMP-deficient mice exhibited a complete lack of cell-cell fusion of osteoclasts and foreign body giant cells (FBGCs), both of which are macrophage-lineage multinuclear cells, although expression of dendritic cell specific transmembrane protein (DC-STAMP), which is also essential for osteoclast/FBGC fusion, was normal. Crossing OC-STAMP-overexpressing transgenic mice with OC-STAMP-deficient mice restored inhibited osteoclast and FBGC cell-cell fusion seen in OC-STAMP-deficient mice. Thus, fusogenic mechanisms in macrophage-lineage cells are regulated via OC-STAMP and DC-STAMP.

Original languageEnglish
Pages (from-to)1289-1297
Number of pages9
JournalJournal of Bone and Mineral Research
Volume27
Issue number6
DOIs
Publication statusPublished - 2012 Jun

Fingerprint

Foreign Body Giant Cells
Cell Fusion
Osteoclasts
Dendritic Cells
Proteins
Macrophages
Transgenic Mice

Keywords

  • CELL-CELL FUSION
  • DC-STAMP
  • DENDRITIC CELL-SPECIFIC TRANSMEMBRANE PROTEIN
  • FBGC
  • OC-STAMP
  • OSTEOCLAST
  • OSTEOCLAST STIMULATORY TRANSMEMBRANE PROTEIN

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Osteoclast stimulatory transmembrane protein and dendritic cell-specific transmembrane protein cooperatively modulate cell-cell fusion to form osteoclasts and foreign body giant cells. / Miyamoto, Hiroya; Suzuki, Takayuki; Miyauchi, Yoshiteru; Iwasaki, Ryotaro; Kobayashi, Tami; Sato, Yuiko; Miyamoto, Kana; Hoshi, Hiroko; Hashimoto, Kazuaki; Yoshida, Shigeyuki; Hao, Wu; Mori, Tomoaki; Kanagawa, Hiroya; Katsuyama, Eri; Fujie, Atsuhiro; Morioka, Hideo; Matsumoto, Morio; Chiba, Kazuhiro; Takeya, Motohiro; Toyama, Yoshiaki; Miyamoto, Takeshi.

In: Journal of Bone and Mineral Research, Vol. 27, No. 6, 06.2012, p. 1289-1297.

Research output: Contribution to journalArticle

Miyamoto, H, Suzuki, T, Miyauchi, Y, Iwasaki, R, Kobayashi, T, Sato, Y, Miyamoto, K, Hoshi, H, Hashimoto, K, Yoshida, S, Hao, W, Mori, T, Kanagawa, H, Katsuyama, E, Fujie, A, Morioka, H, Matsumoto, M, Chiba, K, Takeya, M, Toyama, Y & Miyamoto, T 2012, 'Osteoclast stimulatory transmembrane protein and dendritic cell-specific transmembrane protein cooperatively modulate cell-cell fusion to form osteoclasts and foreign body giant cells', Journal of Bone and Mineral Research, vol. 27, no. 6, pp. 1289-1297. https://doi.org/10.1002/jbmr.1575
Miyamoto, Hiroya ; Suzuki, Takayuki ; Miyauchi, Yoshiteru ; Iwasaki, Ryotaro ; Kobayashi, Tami ; Sato, Yuiko ; Miyamoto, Kana ; Hoshi, Hiroko ; Hashimoto, Kazuaki ; Yoshida, Shigeyuki ; Hao, Wu ; Mori, Tomoaki ; Kanagawa, Hiroya ; Katsuyama, Eri ; Fujie, Atsuhiro ; Morioka, Hideo ; Matsumoto, Morio ; Chiba, Kazuhiro ; Takeya, Motohiro ; Toyama, Yoshiaki ; Miyamoto, Takeshi. / Osteoclast stimulatory transmembrane protein and dendritic cell-specific transmembrane protein cooperatively modulate cell-cell fusion to form osteoclasts and foreign body giant cells. In: Journal of Bone and Mineral Research. 2012 ; Vol. 27, No. 6. pp. 1289-1297.
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AU - Miyamoto, Kana

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AU - Fujie, Atsuhiro

AU - Morioka, Hideo

AU - Matsumoto, Morio

AU - Chiba, Kazuhiro

AU - Takeya, Motohiro

AU - Toyama, Yoshiaki

AU - Miyamoto, Takeshi

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