Osteoclastic activity induces osteomodulin expression in osteoblasts

Ken Ninomiya, Takeshi Miyamoto, Jun ichi Imai, Nobuyuki Fujita, Toru Suzuki, Ryotaro Iwasaki, Mitsuru Yagi, Shinya Watanabe, Yoshiaki Toyama, Toshio Suda

Research output: Contribution to journalArticle

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Abstract

Bone resorption by osteoclasts stimulates bone formation by osteoblasts. To isolate osteoblastic factors coupled with osteoclast activity, we performed microarray and cluster analysis of 8 tissues including bone, and found that among 10,490 genes, osteomodulin (OMD), an extracellular matrix keratan sulfate proteoglycan, was simultaneously induced with osteoclast-specific markers such as MMP9 and Acp5. OMD expression was detected in osteoblasts and upregulated during osteoblast maturation. OMD expression in osteoblasts was also detected immunohistochemically using a specific antibody against OMD. The immunoreactivity against OMD decreased in op/op mice, which lack functional macrophage colony stimulating factor (M-CSF) and are therefore defective in osteoclast formation, when compared to wild-type littermates. OMD expression in op/op mice was upregulated by M-CSF treatment. Since the M-CSF receptor c-Fms was not expressed in osteoblasts, it is likely that OMD is an osteoblast maturation marker that is induced by osteoclast activity.

Original languageEnglish
Pages (from-to)460-466
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume362
Issue number2
DOIs
Publication statusPublished - 2007 Oct 19

Fingerprint

Osteoblasts
Osteoclasts
Bone
Macrophage Colony-Stimulating Factor
Macrophage Colony-Stimulating Factor Receptors
Keratan Sulfate
Cluster analysis
Proteoglycans
Microarray Analysis
Microarrays
Bone Resorption
osteoadherin
Osteogenesis
Extracellular Matrix
Cluster Analysis
Genes
Tissue
Bone and Bones
Antibodies

Keywords

  • Coupling
  • Osteoblast
  • Osteoclast
  • Osteomodulin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Osteoclastic activity induces osteomodulin expression in osteoblasts. / Ninomiya, Ken; Miyamoto, Takeshi; Imai, Jun ichi; Fujita, Nobuyuki; Suzuki, Toru; Iwasaki, Ryotaro; Yagi, Mitsuru; Watanabe, Shinya; Toyama, Yoshiaki; Suda, Toshio.

In: Biochemical and Biophysical Research Communications, Vol. 362, No. 2, 19.10.2007, p. 460-466.

Research output: Contribution to journalArticle

Ninomiya, K, Miyamoto, T, Imai, JI, Fujita, N, Suzuki, T, Iwasaki, R, Yagi, M, Watanabe, S, Toyama, Y & Suda, T 2007, 'Osteoclastic activity induces osteomodulin expression in osteoblasts', Biochemical and Biophysical Research Communications, vol. 362, no. 2, pp. 460-466. https://doi.org/10.1016/j.bbrc.2007.07.193
Ninomiya, Ken ; Miyamoto, Takeshi ; Imai, Jun ichi ; Fujita, Nobuyuki ; Suzuki, Toru ; Iwasaki, Ryotaro ; Yagi, Mitsuru ; Watanabe, Shinya ; Toyama, Yoshiaki ; Suda, Toshio. / Osteoclastic activity induces osteomodulin expression in osteoblasts. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 362, No. 2. pp. 460-466.
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