Osteopontin is an alpha motor neuron marker in the mouse spinal cord

Hidemi Misawa, Mayumi Hara, Shogo Tanabe, Mamiko Niikura, Yasuhiro Moriwaki, Takashi Okuda

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Motor neurons (MNs) are designated as alpha/gamma and fast/slow based on their target sites and the types of muscle fibers innervated; however, few molecular markers that distinguish between these subtypes are available. Here we report that osteopontin (OPN) is a selective marker of alpha MNs in the mouse spinal cord. OPN was detected in approximately 70% of postnatal choline acetyltransferase (ChAT)-positive MNs with relatively large somas, but not in those with smaller somas. OPN +/ChAT + MNs were also positive for NeuN, an alpha MN marker, but were negative for Err3, a gamma MN marker. The size distribution of OPN +/ChAT + cells was nearly identical to that of NeuN +/ChAT + alpha MNs. Group Ia proprioceptive terminals immunoreactive for vesicular glutamate transporter-1 were selectively detected on the OPN +/ChAT + cells. OPN staining was also detected at motor axon terminals at neuromuscular junctions, where the OPN + terminals were positive or negative for SV2A, a marker distinguishing fast/slow motor endplates. Finally, retrograde labeling following intramuscular injection of fast blue indicated that OPN is expressed in both fast and slow MNs. Collectively, our findings show that OPN is an alpha MN marker present in both the soma and the endplates of alpha MNs in the postnatal mouse spinal cord.

Original languageEnglish
Pages (from-to)732-742
Number of pages11
JournalJournal of Neuroscience Research
Volume90
Issue number4
DOIs
Publication statusPublished - 2012 Apr

Fingerprint

Osteopontin
Motor Neurons
Spinal Cord
Choline O-Acetyltransferase
Carisoprodol
Gamma Motor Neurons
Vesicular Glutamate Transport Protein 1
Motor Endplate
Neuromuscular Junction
Intramuscular Injections
Presynaptic Terminals
Staining and Labeling
Muscles

Keywords

  • Alpha motor neuron
  • Fast motor neuron
  • Gamma motor neuron
  • Neuromuscular junction
  • Slow motor neuron

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Osteopontin is an alpha motor neuron marker in the mouse spinal cord. / Misawa, Hidemi; Hara, Mayumi; Tanabe, Shogo; Niikura, Mamiko; Moriwaki, Yasuhiro; Okuda, Takashi.

In: Journal of Neuroscience Research, Vol. 90, No. 4, 04.2012, p. 732-742.

Research output: Contribution to journalArticle

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