Ovariectomy enhances renal cortical expression and function of cyclooxygenase-2

Yuko Tada, Atsuhiro Ichihara, Yukako Koura, Hirokazu Okada, Yuki Kaneshiro, Matsuhiko Hayashi, Takao Saruta

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background. Cyclooxygenase-2 (COX-2) inhibitors are used as analgesics in postmenopausal women, who develop edema and require a salt-restricted diet. This study was performed to determine the renal expression of COX-2 and on COX-2-dependent regulation of renal blood flow (RBF) in ovariectomized rats. Methods. Sprague-Dawley rats were divided into 4 groups: sham-operated rats fed a normal-salt diet (Sh+NS) or a low-salt diet (Sh+LS), and bilaterally ovariectomized rats fed a normal-salt diet (Ox+NS) or a low-salt diet (Ox+LS) (N = 6 in each group). Estrogen replacement therapy was performed on other ovariectomized rats. A renal clearance study was performed in anesthetized animals. Results. Ovariectomy increased renal cortical COX-2 expression independently of dietary salt intake (Sh+NS <Ox+N; Sh+LS <Ox+LS). Inhibition of COX-2 by NS398 reduced the urinary excretion of 6-keto-prostaglandin F1α in all 4 groups, although the reduction was greater in the Ox+LS group than in the Ox+NS and Sh+LS groups, which in turn had a greater reduction than the Sh+NS group. RBF significantly decreased in every group except the Sh+NS group, but no effect on blood pressure, inulin clearance, or urinary sodium excretion was seen. The decrease in RBF was significantly greater in the Ox+LS group than in the Sh+LS and Ox+NS group. The decrease in RBF was dependent on cortical RBF in the Sh+LS and Ox+NS groups, and on both cortical and medullary RBF in the Ox+LS group. Estrogen replacement therapy reversed the ovariectomy-induced changes. Conclusion. Estrogen-dependent COX-2 expression plays an important role in the RBF regulation in female rats.

Original languageEnglish
Pages (from-to)1966-1976
Number of pages11
JournalKidney International
Volume66
Issue number5
DOIs
Publication statusPublished - 2004 Nov
Externally publishedYes

Fingerprint

Renal Circulation
Ovariectomy
Cyclooxygenase 2
Kidney
Salts
Sodium-Restricted Diet
Estrogen Replacement Therapy
Diet
Inulin
Cyclooxygenase 2 Inhibitors
Sprague Dawley Rats
Analgesics
Edema
Estrogens
Sodium
Blood Pressure

Keywords

  • Cells of the thick ascending limb of Henle
  • Cyclooxygenase-2
  • Estrogen replacement therapy
  • Ovariectomy
  • Prostaglandins
  • Renal plasma flow

ASJC Scopus subject areas

  • Nephrology

Cite this

Tada, Y., Ichihara, A., Koura, Y., Okada, H., Kaneshiro, Y., Hayashi, M., & Saruta, T. (2004). Ovariectomy enhances renal cortical expression and function of cyclooxygenase-2. Kidney International, 66(5), 1966-1976. https://doi.org/10.1111/j.1523-1755.2004.00983.x

Ovariectomy enhances renal cortical expression and function of cyclooxygenase-2. / Tada, Yuko; Ichihara, Atsuhiro; Koura, Yukako; Okada, Hirokazu; Kaneshiro, Yuki; Hayashi, Matsuhiko; Saruta, Takao.

In: Kidney International, Vol. 66, No. 5, 11.2004, p. 1966-1976.

Research output: Contribution to journalArticle

Tada, Y, Ichihara, A, Koura, Y, Okada, H, Kaneshiro, Y, Hayashi, M & Saruta, T 2004, 'Ovariectomy enhances renal cortical expression and function of cyclooxygenase-2', Kidney International, vol. 66, no. 5, pp. 1966-1976. https://doi.org/10.1111/j.1523-1755.2004.00983.x
Tada Y, Ichihara A, Koura Y, Okada H, Kaneshiro Y, Hayashi M et al. Ovariectomy enhances renal cortical expression and function of cyclooxygenase-2. Kidney International. 2004 Nov;66(5):1966-1976. https://doi.org/10.1111/j.1523-1755.2004.00983.x
Tada, Yuko ; Ichihara, Atsuhiro ; Koura, Yukako ; Okada, Hirokazu ; Kaneshiro, Yuki ; Hayashi, Matsuhiko ; Saruta, Takao. / Ovariectomy enhances renal cortical expression and function of cyclooxygenase-2. In: Kidney International. 2004 ; Vol. 66, No. 5. pp. 1966-1976.
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abstract = "Background. Cyclooxygenase-2 (COX-2) inhibitors are used as analgesics in postmenopausal women, who develop edema and require a salt-restricted diet. This study was performed to determine the renal expression of COX-2 and on COX-2-dependent regulation of renal blood flow (RBF) in ovariectomized rats. Methods. Sprague-Dawley rats were divided into 4 groups: sham-operated rats fed a normal-salt diet (Sh+NS) or a low-salt diet (Sh+LS), and bilaterally ovariectomized rats fed a normal-salt diet (Ox+NS) or a low-salt diet (Ox+LS) (N = 6 in each group). Estrogen replacement therapy was performed on other ovariectomized rats. A renal clearance study was performed in anesthetized animals. Results. Ovariectomy increased renal cortical COX-2 expression independently of dietary salt intake (Sh+NS <Ox+N; Sh+LS <Ox+LS). Inhibition of COX-2 by NS398 reduced the urinary excretion of 6-keto-prostaglandin F1α in all 4 groups, although the reduction was greater in the Ox+LS group than in the Ox+NS and Sh+LS groups, which in turn had a greater reduction than the Sh+NS group. RBF significantly decreased in every group except the Sh+NS group, but no effect on blood pressure, inulin clearance, or urinary sodium excretion was seen. The decrease in RBF was significantly greater in the Ox+LS group than in the Sh+LS and Ox+NS group. The decrease in RBF was dependent on cortical RBF in the Sh+LS and Ox+NS groups, and on both cortical and medullary RBF in the Ox+LS group. Estrogen replacement therapy reversed the ovariectomy-induced changes. Conclusion. Estrogen-dependent COX-2 expression plays an important role in the RBF regulation in female rats.",
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AU - Ichihara, Atsuhiro

AU - Koura, Yukako

AU - Okada, Hirokazu

AU - Kaneshiro, Yuki

AU - Hayashi, Matsuhiko

AU - Saruta, Takao

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N2 - Background. Cyclooxygenase-2 (COX-2) inhibitors are used as analgesics in postmenopausal women, who develop edema and require a salt-restricted diet. This study was performed to determine the renal expression of COX-2 and on COX-2-dependent regulation of renal blood flow (RBF) in ovariectomized rats. Methods. Sprague-Dawley rats were divided into 4 groups: sham-operated rats fed a normal-salt diet (Sh+NS) or a low-salt diet (Sh+LS), and bilaterally ovariectomized rats fed a normal-salt diet (Ox+NS) or a low-salt diet (Ox+LS) (N = 6 in each group). Estrogen replacement therapy was performed on other ovariectomized rats. A renal clearance study was performed in anesthetized animals. Results. Ovariectomy increased renal cortical COX-2 expression independently of dietary salt intake (Sh+NS <Ox+N; Sh+LS <Ox+LS). Inhibition of COX-2 by NS398 reduced the urinary excretion of 6-keto-prostaglandin F1α in all 4 groups, although the reduction was greater in the Ox+LS group than in the Ox+NS and Sh+LS groups, which in turn had a greater reduction than the Sh+NS group. RBF significantly decreased in every group except the Sh+NS group, but no effect on blood pressure, inulin clearance, or urinary sodium excretion was seen. The decrease in RBF was significantly greater in the Ox+LS group than in the Sh+LS and Ox+NS group. The decrease in RBF was dependent on cortical RBF in the Sh+LS and Ox+NS groups, and on both cortical and medullary RBF in the Ox+LS group. Estrogen replacement therapy reversed the ovariectomy-induced changes. Conclusion. Estrogen-dependent COX-2 expression plays an important role in the RBF regulation in female rats.

AB - Background. Cyclooxygenase-2 (COX-2) inhibitors are used as analgesics in postmenopausal women, who develop edema and require a salt-restricted diet. This study was performed to determine the renal expression of COX-2 and on COX-2-dependent regulation of renal blood flow (RBF) in ovariectomized rats. Methods. Sprague-Dawley rats were divided into 4 groups: sham-operated rats fed a normal-salt diet (Sh+NS) or a low-salt diet (Sh+LS), and bilaterally ovariectomized rats fed a normal-salt diet (Ox+NS) or a low-salt diet (Ox+LS) (N = 6 in each group). Estrogen replacement therapy was performed on other ovariectomized rats. A renal clearance study was performed in anesthetized animals. Results. Ovariectomy increased renal cortical COX-2 expression independently of dietary salt intake (Sh+NS <Ox+N; Sh+LS <Ox+LS). Inhibition of COX-2 by NS398 reduced the urinary excretion of 6-keto-prostaglandin F1α in all 4 groups, although the reduction was greater in the Ox+LS group than in the Ox+NS and Sh+LS groups, which in turn had a greater reduction than the Sh+NS group. RBF significantly decreased in every group except the Sh+NS group, but no effect on blood pressure, inulin clearance, or urinary sodium excretion was seen. The decrease in RBF was significantly greater in the Ox+LS group than in the Sh+LS and Ox+NS group. The decrease in RBF was dependent on cortical RBF in the Sh+LS and Ox+NS groups, and on both cortical and medullary RBF in the Ox+LS group. Estrogen replacement therapy reversed the ovariectomy-induced changes. Conclusion. Estrogen-dependent COX-2 expression plays an important role in the RBF regulation in female rats.

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KW - Prostaglandins

KW - Renal plasma flow

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