Over-expression of Aurora-A targets cytoplasmic polyadenylation element binding protein and promotes mRNA polyadenylation of Cdk1 and cyclin B1

Takashi Sasayama, Tomotoshi Marumoto, Naoko Kunitoku, Dongwei Zhang, Norihiko Tamaki, Eiji Kohmura, Hideyuki Saya, Toru Hirota

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Aurora-A is a centrosomal serine-threonine kinase that regulates mitosis. Over-expression of Aurora-A has been found in a wide range of tumors and has been implicated in oncogenic transformation. However, how Aurora-A over-expression contributes to promotion of carcinogenesis remains elusive. Immunohistochemical analysis of breast tumors revealed that over-expressed Aurora-A is not restricted to the centrosomes but is also found in the cytoplasm. This over-expressed Aurora-A appeared to be phosphorylated on Thr288, which is known to be required for its enzymatic activation. In analogy to Aurora-A's role in oocyte maturation and the early embryonic cell cycle, here we investigated whether ectopically over-expressed Aurora-A can similarly stimulate polyadenylation of mRNA in human somatic cultured cells by interacting with a human ortholog of cytoplasmic polyadenylation element binding protein, h-CPEB. In vitro experiments revealed that Aurora-A binds directly to, and phosphorylates, h-CPEB.We found that polyadenylation of mRNA tails of cyclin B1 and Cdk1 was synergistically stimulated when Aurora-A and h-CPEB were overexpressed, and they were further promoted in the presence of an Aurora-A activator Ajuba. Our results suggest a function of ectopically over-expressed Aurora-A that might be relevant for carcinogenesis.

Original languageEnglish
Pages (from-to)627-638
Number of pages12
JournalGenes to Cells
Volume10
Issue number7
DOIs
Publication statusPublished - 2005 Jul
Externally publishedYes

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Cyclin B1
Polyadenylation
Carrier Proteins
Messenger RNA
Carcinogenesis
Centrosome
Protein-Serine-Threonine Kinases
Mitosis
Oocytes
Tail
Cultured Cells
Cell Cycle
Cytoplasm
Breast Neoplasms
Neoplasms

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Over-expression of Aurora-A targets cytoplasmic polyadenylation element binding protein and promotes mRNA polyadenylation of Cdk1 and cyclin B1. / Sasayama, Takashi; Marumoto, Tomotoshi; Kunitoku, Naoko; Zhang, Dongwei; Tamaki, Norihiko; Kohmura, Eiji; Saya, Hideyuki; Hirota, Toru.

In: Genes to Cells, Vol. 10, No. 7, 07.2005, p. 627-638.

Research output: Contribution to journalArticle

Sasayama, Takashi ; Marumoto, Tomotoshi ; Kunitoku, Naoko ; Zhang, Dongwei ; Tamaki, Norihiko ; Kohmura, Eiji ; Saya, Hideyuki ; Hirota, Toru. / Over-expression of Aurora-A targets cytoplasmic polyadenylation element binding protein and promotes mRNA polyadenylation of Cdk1 and cyclin B1. In: Genes to Cells. 2005 ; Vol. 10, No. 7. pp. 627-638.
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