Abstract
The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwent apoptosis, which was not associated with G1-arrest. Cdk4 kinase activity was significantly higher in cdk4-, or cyclin D1-expressing cells. Furthermore, induction of apoptosis by cdk4 was abrogated by co-transfection of p16INK4, or dominant negative cdk4. These results suggest that upregulation of cdk4 kinase activity is a primary and critical mediator of apoptosis in PC12 cells under physiological conditions.
Original language | English |
---|---|
Pages (from-to) | 382-388 |
Number of pages | 7 |
Journal | FEBS Letters |
Volume | 509 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2001 Dec 14 |
Externally published | Yes |
Keywords
- Apoptosis
- Cdk inhibitor
- Cdk4
- Cyclin D1
- Dominant negative cdk4
- PC12 cell
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology