Abstract
The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwent apoptosis, which was not associated with G1-arrest. Cdk4 kinase activity was significantly higher in cdk4-, or cyclin D1-expressing cells. Furthermore, induction of apoptosis by cdk4 was abrogated by co-transfection of p16INK4, or dominant negative cdk4. These results suggest that upregulation of cdk4 kinase activity is a primary and critical mediator of apoptosis in PC12 cells under physiological conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 382-388 |
| Number of pages | 7 |
| Journal | FEBS Letters |
| Volume | 509 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2001 Dec 14 |
| Externally published | Yes |
Fingerprint
Keywords
- Apoptosis
- Cdk inhibitor
- Cdk4
- Cyclin D1
- Dominant negative cdk4
- PC12 cell
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support. / Katayama, Kazuhiro; Dobashi, Yoh; Kitagawa, Masatoshi; Kamekura, Soichiro; Kawai, Masataka; Kadoya, Yuichi; Kameya, Toru.
In: FEBS Letters, Vol. 509, No. 3, 14.12.2001, p. 382-388.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support
AU - Katayama, Kazuhiro
AU - Dobashi, Yoh
AU - Kitagawa, Masatoshi
AU - Kamekura, Soichiro
AU - Kawai, Masataka
AU - Kadoya, Yuichi
AU - Kameya, Toru
PY - 2001/12/14
Y1 - 2001/12/14
N2 - The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwent apoptosis, which was not associated with G1-arrest. Cdk4 kinase activity was significantly higher in cdk4-, or cyclin D1-expressing cells. Furthermore, induction of apoptosis by cdk4 was abrogated by co-transfection of p16INK4, or dominant negative cdk4. These results suggest that upregulation of cdk4 kinase activity is a primary and critical mediator of apoptosis in PC12 cells under physiological conditions.
AB - The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwent apoptosis, which was not associated with G1-arrest. Cdk4 kinase activity was significantly higher in cdk4-, or cyclin D1-expressing cells. Furthermore, induction of apoptosis by cdk4 was abrogated by co-transfection of p16INK4, or dominant negative cdk4. These results suggest that upregulation of cdk4 kinase activity is a primary and critical mediator of apoptosis in PC12 cells under physiological conditions.
KW - Apoptosis
KW - Cdk inhibitor
KW - Cdk4
KW - Cyclin D1
KW - Dominant negative cdk4
KW - PC12 cell
UR - http://www.scopus.com/inward/record.url?scp=0035861852&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035861852&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(01)03200-8
DO - 10.1016/S0014-5793(01)03200-8
M3 - Article
C2 - 11749960
AN - SCOPUS:0035861852
VL - 509
SP - 382
EP - 388
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -