Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support

Kazuhiro Katayama, Yoh Dobashi, Masatoshi Kitagawa, Soichiro Kamekura, Masataka Kawai, Yuichi Kadoya, Toru Kameya

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwent apoptosis, which was not associated with G1-arrest. Cdk4 kinase activity was significantly higher in cdk4-, or cyclin D1-expressing cells. Furthermore, induction of apoptosis by cdk4 was abrogated by co-transfection of p16INK4, or dominant negative cdk4. These results suggest that upregulation of cdk4 kinase activity is a primary and critical mediator of apoptosis in PC12 cells under physiological conditions.

Original languageEnglish
Pages (from-to)382-388
Number of pages7
JournalFEBS Letters
Volume509
Issue number3
DOIs
Publication statusPublished - 2001 Dec 14
Externally publishedYes

Fingerprint

PC12 Cells
Cyclin D1
Apoptosis
Phosphotransferases
Cyclins
Cyclin-Dependent Kinases
Flow cytometry
Pheochromocytoma
Fluorescent Antibody Technique
Transfection
Rats
Cell Cycle
Flow Cytometry
Up-Regulation
Cells
Molecules
Growth

Keywords

  • Apoptosis
  • Cdk inhibitor
  • Cdk4
  • Cyclin D1
  • Dominant negative cdk4
  • PC12 cell

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Katayama, K., Dobashi, Y., Kitagawa, M., Kamekura, S., Kawai, M., Kadoya, Y., & Kameya, T. (2001). Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support. FEBS Letters, 509(3), 382-388. https://doi.org/10.1016/S0014-5793(01)03200-8

Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support. / Katayama, Kazuhiro; Dobashi, Yoh; Kitagawa, Masatoshi; Kamekura, Soichiro; Kawai, Masataka; Kadoya, Yuichi; Kameya, Toru.

In: FEBS Letters, Vol. 509, No. 3, 14.12.2001, p. 382-388.

Research output: Contribution to journalArticle

Katayama, K, Dobashi, Y, Kitagawa, M, Kamekura, S, Kawai, M, Kadoya, Y & Kameya, T 2001, 'Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support', FEBS Letters, vol. 509, no. 3, pp. 382-388. https://doi.org/10.1016/S0014-5793(01)03200-8
Katayama K, Dobashi Y, Kitagawa M, Kamekura S, Kawai M, Kadoya Y et al. Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support. FEBS Letters. 2001 Dec 14;509(3):382-388. https://doi.org/10.1016/S0014-5793(01)03200-8
Katayama, Kazuhiro ; Dobashi, Yoh ; Kitagawa, Masatoshi ; Kamekura, Soichiro ; Kawai, Masataka ; Kadoya, Yuichi ; Kameya, Toru. / Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support. In: FEBS Letters. 2001 ; Vol. 509, No. 3. pp. 382-388.
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