Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support

Kazuhiro Katayama; Yoh Dobashi; Masatoshi Kitagawa; Soichiro Kamekura; Masataka Kawai; Yuichi Kadoya; Toru Kameya

doi:10.1016/S0014-5793(01)03200-8

Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support

Kazuhiro Katayama, Yoh Dobashi, Masatoshi Kitagawa, Soichiro Kamekura, Masataka Kawai, Yuichi Kadoya, Toru Kameya

School of Pharmacy

Research output: Contribution to journal › Article

18 Citations (Scopus)

Abstract

The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwent apoptosis, which was not associated with G1-arrest. Cdk4 kinase activity was significantly higher in cdk4-, or cyclin D1-expressing cells. Furthermore, induction of apoptosis by cdk4 was abrogated by co-transfection of p16^INK4, or dominant negative cdk4. These results suggest that upregulation of cdk4 kinase activity is a primary and critical mediator of apoptosis in PC12 cells under physiological conditions.

Original language	English
Pages (from-to)	382-388
Number of pages	7
Journal	FEBS Letters
Volume	509
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(01)03200-8
Publication status	Published - 2001 Dec 14

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Keywords

Apoptosis
Cdk inhibitor
Cdk4
Cyclin D1
Dominant negative cdk4
PC12 cell

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Cite this

Katayama, K., Dobashi, Y., Kitagawa, M., Kamekura, S., Kawai, M., Kadoya, Y., & Kameya, T. (2001). Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support. FEBS Letters, 509(3), 382-388. https://doi.org/10.1016/S0014-5793(01)03200-8

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abstract = "The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwent apoptosis, which was not associated with G1-arrest. Cdk4 kinase activity was significantly higher in cdk4-, or cyclin D1-expressing cells. Furthermore, induction of apoptosis by cdk4 was abrogated by co-transfection of p16INK4, or dominant negative cdk4. These results suggest that upregulation of cdk4 kinase activity is a primary and critical mediator of apoptosis in PC12 cells under physiological conditions.",

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AU - Kadoya, Yuichi

AU - Kameya, Toru

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Access to Document

10.1016/S0014-5793(01)03200-8