Overexpression of cyclase-associated protein 2 in multistage hepatocarcinogenesis

Rie Shibata, Taisuke Mori, Wenlin Du, Makoto Chuma, Masahiro Gotoh, Motohide Shimazu, Masakazu Ueda, Setsuo Hirohashi, Michiie Sakamoto

Research output: Contribution to journalArticle

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Abstract

Purpose: Hepatocellular carcinoma (HCC) associated with chronic liver disease is known to show an obvious multistage process of tumor progression. We previously identified heat shock protein 70 as a molecular marker of early HCC during investigation of expression profiling in multistage hepatocarcinogenesis. In this report, we examined cyclase-associated protein 2 (CAP2), which is also listed as an up-regulated gene in early HCC. Experimental Design: We measured the level of CAP2 mRNA by real-time quantitative PCR. We raised a polyclonal antibody against CAP2 and we confirmed the expression of CAP2 by immunoblotting and immunohistochemistry in HCC cell lines and HCC tissues. Results: According to real-time quantitative PCR, the level of CAP2 mRNA was up-regulated in early HCC when compared with noncancerous liver tissue, and it was further up-regulated in progressed HCC. We raised a polyclonal antibody against CAP2, which showed a single 53-kDa band of strong intensity in the human HCC cell lines and HCC tissues but only a weak band in the noncancerous liver tissues in Western blot analysis. Immunohistochemical examination of CAP2 revealed its significant overexpression in early HCC when compared with noncancerous and precancerous lesions and in progressed HCC when compared with early HCC. Conclusion: Our findings show that CAP2 is up-regulated in HCC when compared with noncancerous and precancerous lesions. This is the first report that proves that CAP2 is up-regulated in human cancers and that this is possibly related to multistage hepatocarcinogenesis.

Original languageEnglish
Pages (from-to)5363-5368
Number of pages6
JournalClinical Cancer Research
Volume12
Issue number18
DOIs
Publication statusPublished - 2006 Sep 15

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Hepatocellular Carcinoma
Proteins
Real-Time Polymerase Chain Reaction
Cell Line
Messenger RNA
HSP70 Heat-Shock Proteins
Antibodies
Liver
Immunoblotting
Liver Diseases
Neoplasms
Chronic Disease
Research Design
Western Blotting
Immunohistochemistry

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Overexpression of cyclase-associated protein 2 in multistage hepatocarcinogenesis. / Shibata, Rie; Mori, Taisuke; Du, Wenlin; Chuma, Makoto; Gotoh, Masahiro; Shimazu, Motohide; Ueda, Masakazu; Hirohashi, Setsuo; Sakamoto, Michiie.

In: Clinical Cancer Research, Vol. 12, No. 18, 15.09.2006, p. 5363-5368.

Research output: Contribution to journalArticle

Shibata, R, Mori, T, Du, W, Chuma, M, Gotoh, M, Shimazu, M, Ueda, M, Hirohashi, S & Sakamoto, M 2006, 'Overexpression of cyclase-associated protein 2 in multistage hepatocarcinogenesis', Clinical Cancer Research, vol. 12, no. 18, pp. 5363-5368. https://doi.org/10.1158/1078-0432.CCR-05-2245
Shibata, Rie ; Mori, Taisuke ; Du, Wenlin ; Chuma, Makoto ; Gotoh, Masahiro ; Shimazu, Motohide ; Ueda, Masakazu ; Hirohashi, Setsuo ; Sakamoto, Michiie. / Overexpression of cyclase-associated protein 2 in multistage hepatocarcinogenesis. In: Clinical Cancer Research. 2006 ; Vol. 12, No. 18. pp. 5363-5368.
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