Overexpression of granulocyte colony-stimulating factor in vivo decreases the level of polyploidization of mouse bone marrow megakaryocytes

Masahiro Saito, Koji Takada, Taketo Yamada, Junichiro Fujimoto

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The in vivo effect of G-CSF on the maturation of mouse bone marrow megakaryocytes was studied by monitoring the DNA contents. Megakaryocytes were first identified by a specific 1C2 monoclonal antibody against mouse platelets and megakaryocytes and DNA contents of these cells were measured by propidium iodine. Megakaryocytes of mice transgenic for human G-CSF had a modal DNA class of 8N, showing a striking contrast to the previous reports that normal mouse megakaryocytes from most strains have 16N DNA content as a modal class. Daily 10 μg administration of G-CSF to mice for three to five days affected the DNA distribution pattern of bone marrow megakaryocytes, with a higher proportion of cells having 8N DNA contents. This G-CSF treatment, however, did not influence the peripheral blood platelet count or bone marrow megakaryocyte number. Administration of G-CSF along with thrombopoietin (TPO) reduced the proportion of megakaryocytes, with 32N DNA, the DNA class that was increased by TPO. Finally, the presence of mRNA for the mouse G-CSF receptor was demonstrated in two megakaryoblastic cell lines by reverse transcriptase polymerase chain reaction. These results indicated that G-CSF may have a suppressive effect on the maturation of mouse bone marrow megakaryocytes when monitored by the DNA polyploidy. Although further study is clearly necessary, the presence of mRNA for the G-CSF receptor in megakaryocytic lineage strongly suggests the direct action of G-CSF on this cell lineage. Stem Cells.

Original languageEnglish
Pages (from-to)124-131
Number of pages8
JournalStem Cells
Volume14
Issue number1
Publication statusPublished - 1996

Fingerprint

Megakaryocytes
Granulocyte Colony-Stimulating Factor
Bone Marrow
DNA
Granulocyte Colony-Stimulating Factor Receptors
Thrombopoietin
Messenger RNA
Polyploidy
Propidium
Cell Lineage
Reverse Transcriptase Polymerase Chain Reaction
Platelet Count
Iodine
Transgenic Mice
Stem Cells
Blood Platelets
Monoclonal Antibodies
Cell Line

Keywords

  • DNA
  • G-CSF
  • Megakaryocyte
  • Megakaryocytopoiesis
  • Ploidy
  • TPO

ASJC Scopus subject areas

  • Cell Biology

Cite this

Overexpression of granulocyte colony-stimulating factor in vivo decreases the level of polyploidization of mouse bone marrow megakaryocytes. / Saito, Masahiro; Takada, Koji; Yamada, Taketo; Fujimoto, Junichiro.

In: Stem Cells, Vol. 14, No. 1, 1996, p. 124-131.

Research output: Contribution to journalArticle

Saito, Masahiro ; Takada, Koji ; Yamada, Taketo ; Fujimoto, Junichiro. / Overexpression of granulocyte colony-stimulating factor in vivo decreases the level of polyploidization of mouse bone marrow megakaryocytes. In: Stem Cells. 1996 ; Vol. 14, No. 1. pp. 124-131.
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