Overexpression of leucine-rich repeat-containing G protein-coupled receptor 5 in colorectal cancer

Hiroshi Uchida, Ken Yamazaki, Mariko Fukuma, Taketo Yamada, Tetsu Hayashida, Hirotoshi Hasegawa, Masaki Kitajima, Yuukou Kitagawa, Michiie Sakamoto

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) is a 7-transmembrane receptor reportedly expressed in stem cells of the intestinal crypts and hair follicles of mice. Overexpression of LGR5 is observed in some types of cancer; however, there has been no specific assessment in colorectal tumorigenesis. We performed quantitative RT-PCR for LGR5 expression in 37 representative cancer cell lines, and showed that LGR5 mRNA was frequently overexpressed in colon cancer cell lines. Moreover, LGR5 expression was higher in colon cancer cell lines derived from metastatic tumors compared with those from primary tumors. In clinical specimens, there was significant overexpression of LGR5 in 35 of 50 colorectal cancers (CRCs), and in seven of seven sporadic colonic adenomas, compared with matched normal mucosa. This suggests up-regulation of LGR5 from the early stage of colorectal tumorigenesis. LGR5 expression showed marked variation among CRC cases and correlated significantly with lymphatic invasion, vascular invasion, tumor depth, lymph node metastasis, and tumor stage (IIIC vs. IIIB). In addition to cancer cells, crypt base columnar cells of the small intestine and colon were shown by in situ hybridization to express LGR5. This is the first report suggesting the involvement of LGR5, not only in early events but also in late events in colorectal tumorigenesis.

Original languageEnglish
Pages (from-to)1731-1737
Number of pages7
JournalCancer Science
Volume101
Issue number7
DOIs
Publication statusPublished - 2010 Jul

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G-Protein-Coupled Receptors
Leucine
Colorectal Neoplasms
Neoplasms
Carcinogenesis
Cell Line
Colonic Neoplasms
Hair Follicle
Adenoma
Small Intestine
In Situ Hybridization
Blood Vessels
Colon
Mucous Membrane
Up-Regulation
Stem Cells
Lymph Nodes
Neoplasm Metastasis
Polymerase Chain Reaction
Messenger RNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Overexpression of leucine-rich repeat-containing G protein-coupled receptor 5 in colorectal cancer. / Uchida, Hiroshi; Yamazaki, Ken; Fukuma, Mariko; Yamada, Taketo; Hayashida, Tetsu; Hasegawa, Hirotoshi; Kitajima, Masaki; Kitagawa, Yuukou; Sakamoto, Michiie.

In: Cancer Science, Vol. 101, No. 7, 07.2010, p. 1731-1737.

Research output: Contribution to journalArticle

Uchida, Hiroshi ; Yamazaki, Ken ; Fukuma, Mariko ; Yamada, Taketo ; Hayashida, Tetsu ; Hasegawa, Hirotoshi ; Kitajima, Masaki ; Kitagawa, Yuukou ; Sakamoto, Michiie. / Overexpression of leucine-rich repeat-containing G protein-coupled receptor 5 in colorectal cancer. In: Cancer Science. 2010 ; Vol. 101, No. 7. pp. 1731-1737.
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