Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells

Takayuki Mitsuhashi, Yoko Aoki, Yaman Z. Eksioglu, Takao Takahashi, Pradeep G. Bhide, Steven A. Reeves, Verne S. Caviness

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

We describe a mouse model in which p27Kip1 transgene expression is spatially restricted to the central nervous system neuroepithelium and temporally controlled with doxycycline. Transgene-specific transcripts are detectable within 6 h of doxycycline administration, and maximum nonlethal expression is approached within 12 h. After 18-26 h of transgene expression, the G1 phase of the cell cycle is estimated to increase from 9 to 13 h in the neocortical neuroepithelium, the maximum G1 phase length attainable in this proliferative population in normal mice. Thus our data establish a direct link between p27Kip1 and control of G1 phase length in the mammalian central nervous system and unveil intrinsic mechanisms that constrain the G1 phase length to a putative physiological maximum despite ongoing p27Kip1 transgene expression.

Original languageEnglish
Pages (from-to)6435-6440
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number11
DOIs
Publication statusPublished - 2001 May 22

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G1 Phase
Transgenes
Stem Cells
Central Nervous System
Gene Expression
Doxycycline
Cell Cycle
Population

ASJC Scopus subject areas

  • Genetics
  • General

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Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells. / Mitsuhashi, Takayuki; Aoki, Yoko; Eksioglu, Yaman Z.; Takahashi, Takao; Bhide, Pradeep G.; Reeves, Steven A.; Caviness, Verne S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 11, 22.05.2001, p. 6435-6440.

Research output: Contribution to journalArticle

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