Abstract
Malignant melanoma is the most aggressive neoplasm, with severe metastatic potential. microRNAs represent a class of endogenously expressed, small non-coding RNAs that regulate gene expression. As a consequence, the translation of these mRNAs is inhibited or they are destabilized resulting in downregulation of the encoded protein. The microRNA-34 (miR-34) family, which comprises three processed microRNAs (miR-34a/b/c) was identified as the mediator of tumor suppression by p53. Many reports suggest that the miR-34s contribute to the inhibition of invasion or metastasis in various tumor types. In this study, we evaluated the expression of the miR-34 family in four human melanoma cell lines (A375, G361, C32TG and SK-MEL-24) which have the wild-type p53 gene using real-time reverse transcription PCR. We also examined their generative and invasive characteristics using the cell proliferation assay and the invasion/migration assay, respectively. All four melanoma cell lines showed significant expression of miR-34s - A375: miR-34a 0.6176, miR-34b 0.7625, miR-34c 0.7877; G361: 7.6424, 16.4127, 22.0332; C32TG: 2.1630, 2.1091, 8.4425; SK-MEL-24: 0.3621, 2.5659, 8.5907. The cell doubling times of these cell lines in h:min were as follows: A375 23:23, G361 68:24, C32TG 47:22 and SK-MEL-24 67:03. The in vitro generation times of G361 and SK-MEL-24, which showed increased expression of miR-34c, were significantly shorter than A375 with decreased expression of miR-34c (p=0.0063, ANOVA). Invasion (%) of the four cell lines was as follows: A375 44.0%, G361 22.4%, C32TG 13.8% and SK-MEL-24 45.0%. In vitro invasiveness of G361 and C32TG, which showed increased expression of miR-34a, was significantly suppressed (p=0.005, ANOVA). These results suggest that overexpression of miR-34a and c suppresses invasive and generative potentials, respectively, in human malignant melanoma.
| Original language | English |
|---|---|
| Pages (from-to) | 793-796 |
| Number of pages | 4 |
| Journal | Experimental and Therapeutic Medicine |
| Volume | 3 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2012 May |
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Keywords
- Melanoma
- MicroRNA-34
- p53
ASJC Scopus subject areas
- Medicine(all)
- Cancer Research
- Immunology and Microbiology (miscellaneous)
Cite this
Overexpression of the miR-34 family suppresses invasive growth of malignant melanoma with the wild-type p53 gene. / Yamazaki, Hitoshi; Chijiwa, Tsuyoshi; Inoue, Yoshimasa; Abe, Yoshiyuki; Suemizu, Hiroshi; Kawai, Kenji; Wakui, Masatoshi; Furukawa, Daisuke; Mukai, Masaya; Kuwao, Sadahito; Saegusa, Makoto; Nakamura, Masato.
In: Experimental and Therapeutic Medicine, Vol. 3, No. 5, 05.2012, p. 793-796.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Overexpression of the miR-34 family suppresses invasive growth of malignant melanoma with the wild-type p53 gene
AU - Yamazaki, Hitoshi
AU - Chijiwa, Tsuyoshi
AU - Inoue, Yoshimasa
AU - Abe, Yoshiyuki
AU - Suemizu, Hiroshi
AU - Kawai, Kenji
AU - Wakui, Masatoshi
AU - Furukawa, Daisuke
AU - Mukai, Masaya
AU - Kuwao, Sadahito
AU - Saegusa, Makoto
AU - Nakamura, Masato
PY - 2012/5
Y1 - 2012/5
N2 - Malignant melanoma is the most aggressive neoplasm, with severe metastatic potential. microRNAs represent a class of endogenously expressed, small non-coding RNAs that regulate gene expression. As a consequence, the translation of these mRNAs is inhibited or they are destabilized resulting in downregulation of the encoded protein. The microRNA-34 (miR-34) family, which comprises three processed microRNAs (miR-34a/b/c) was identified as the mediator of tumor suppression by p53. Many reports suggest that the miR-34s contribute to the inhibition of invasion or metastasis in various tumor types. In this study, we evaluated the expression of the miR-34 family in four human melanoma cell lines (A375, G361, C32TG and SK-MEL-24) which have the wild-type p53 gene using real-time reverse transcription PCR. We also examined their generative and invasive characteristics using the cell proliferation assay and the invasion/migration assay, respectively. All four melanoma cell lines showed significant expression of miR-34s - A375: miR-34a 0.6176, miR-34b 0.7625, miR-34c 0.7877; G361: 7.6424, 16.4127, 22.0332; C32TG: 2.1630, 2.1091, 8.4425; SK-MEL-24: 0.3621, 2.5659, 8.5907. The cell doubling times of these cell lines in h:min were as follows: A375 23:23, G361 68:24, C32TG 47:22 and SK-MEL-24 67:03. The in vitro generation times of G361 and SK-MEL-24, which showed increased expression of miR-34c, were significantly shorter than A375 with decreased expression of miR-34c (p=0.0063, ANOVA). Invasion (%) of the four cell lines was as follows: A375 44.0%, G361 22.4%, C32TG 13.8% and SK-MEL-24 45.0%. In vitro invasiveness of G361 and C32TG, which showed increased expression of miR-34a, was significantly suppressed (p=0.005, ANOVA). These results suggest that overexpression of miR-34a and c suppresses invasive and generative potentials, respectively, in human malignant melanoma.
AB - Malignant melanoma is the most aggressive neoplasm, with severe metastatic potential. microRNAs represent a class of endogenously expressed, small non-coding RNAs that regulate gene expression. As a consequence, the translation of these mRNAs is inhibited or they are destabilized resulting in downregulation of the encoded protein. The microRNA-34 (miR-34) family, which comprises three processed microRNAs (miR-34a/b/c) was identified as the mediator of tumor suppression by p53. Many reports suggest that the miR-34s contribute to the inhibition of invasion or metastasis in various tumor types. In this study, we evaluated the expression of the miR-34 family in four human melanoma cell lines (A375, G361, C32TG and SK-MEL-24) which have the wild-type p53 gene using real-time reverse transcription PCR. We also examined their generative and invasive characteristics using the cell proliferation assay and the invasion/migration assay, respectively. All four melanoma cell lines showed significant expression of miR-34s - A375: miR-34a 0.6176, miR-34b 0.7625, miR-34c 0.7877; G361: 7.6424, 16.4127, 22.0332; C32TG: 2.1630, 2.1091, 8.4425; SK-MEL-24: 0.3621, 2.5659, 8.5907. The cell doubling times of these cell lines in h:min were as follows: A375 23:23, G361 68:24, C32TG 47:22 and SK-MEL-24 67:03. The in vitro generation times of G361 and SK-MEL-24, which showed increased expression of miR-34c, were significantly shorter than A375 with decreased expression of miR-34c (p=0.0063, ANOVA). Invasion (%) of the four cell lines was as follows: A375 44.0%, G361 22.4%, C32TG 13.8% and SK-MEL-24 45.0%. In vitro invasiveness of G361 and C32TG, which showed increased expression of miR-34a, was significantly suppressed (p=0.005, ANOVA). These results suggest that overexpression of miR-34a and c suppresses invasive and generative potentials, respectively, in human malignant melanoma.
KW - Melanoma
KW - MicroRNA-34
KW - p53
UR - http://www.scopus.com/inward/record.url?scp=84858636221&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84858636221&partnerID=8YFLogxK
U2 - 10.3892/etm.2012.497
DO - 10.3892/etm.2012.497
M3 - Article
AN - SCOPUS:84858636221
VL - 3
SP - 793
EP - 796
JO - Experimental and Therapeutic Medicine
JF - Experimental and Therapeutic Medicine
SN - 1792-0981
IS - 5
ER -