Oxidative stress induces GSTP1 and CYP3A4 expression in the human erythroleukemia cell line, K562

Fusako Nagai, Eriko Kato, Hiroomi Tamura

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Abstract

To explore the effects of oxidative stress on metabolizing enzymes in blood cells, we examined the effect of hydrogen peroxide (H2O2) on glutathione S-transferase (GST) and cytochrome P450 (CYP) expression in a human erythroleukemic cell line, K562. After adding H2O2 (up to 100 μM) to the culture medium of K562 cells, the expression levels of GST and CYP enzymes were monitored by RT-PCR. The expression of GSTP1 and CYP3A4 was induced by oxidative stress. Quantitative PCR and immunoblot analysis revealed a 3- to 4-fold increase in GSTP1 and CYP3A4 mRNA, and a 2- to 3-fold increase in GSTP1 and CYP3A4 protein levels, 3 d after the addition of 100 μM H2O2. Induction was H2O2 dose-dependent and also depended on the length of culture. Our results suggest that oxidative stress may affect GST and/or CYP expression in human blood cells, which may alter the metabolism of drugs and xenobiotics and thus, the toxicity of these compounds to the blood cells.

Original languageEnglish
Pages (from-to)492-495
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume27
Issue number4
DOIs
Publication statusPublished - 2004 Apr

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Keywords

  • CYP3A4
  • GSTP1
  • Induction
  • K562
  • Oxidative stress

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

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