Oxidative stress suppresses the endothelial secretion of endothelin

T. Saito, H. Itoh, T. Chun, T. Igaki, Y. Mori, J. Yamashita, K. Doi, T. Tanaka, M. Inoue, K. Masatsugu, Y. Fukunaga, N. Sawada, K. Tojo, Y. Saito, T. Hosoya, K. Nakao

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17 Citations (Scopus)

Abstract

To address endothelial function on vascular walls exposed to oxidative stress, we investigated the effect of oxidative stress on the secretion of endothelin-1 (ET-1) from cultured bovine carotid artery endothelial cells (BAECs). Concentrations of ET-1 in the media were measured by a specific radioimmunoassay and ET-1 mRNA expression was estimated by Northern blot analysis. Treatment of BAECs with 0.5-2.0 mM H2O2 for 3 h suppressed both ET-1 secretion and ET-1 mRNA expression in a dose-dependent manner compared to control. Attenuation of ET-1 mRNA expression by H2O2 was revealed to take place at the transcriptional level. The addition of NG-nitro-L-arginine-methyl ester (L-NAME) 10 μm, a specific nitric oxide synthase inhibitor, had no effect on H2O2-induced suppression of ET-1 mRNA expression. Suppression of ET secretion under oxidative stress observed in the present study is proposed to be a compensatory mechanism of endothelial cells to inhibit vasoconstriction and proliferation during oxidative stress.

Original languageEnglish
Pages (from-to)S345-S347
JournalJournal of Cardiovascular Pharmacology
Volume31
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 1998 Jan 1
Externally publishedYes

Keywords

  • Endothelial cells
  • Endothelin
  • Hydrogen peroxide
  • L-NAME
  • Oxidative stress

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Saito, T., Itoh, H., Chun, T., Igaki, T., Mori, Y., Yamashita, J., Doi, K., Tanaka, T., Inoue, M., Masatsugu, K., Fukunaga, Y., Sawada, N., Tojo, K., Saito, Y., Hosoya, T., & Nakao, K. (1998). Oxidative stress suppresses the endothelial secretion of endothelin. Journal of Cardiovascular Pharmacology, 31(SUPPL. 1), S345-S347. https://doi.org/10.1097/00005344-199800001-00096