Oxidative stress suppresses the endothelial secretion of endothelin

T. Saito, Hiroshi Itoh, T. Chun, T. Igaki, Y. Mori, J. Yamashita, K. Doi, T. Tanaka, M. Inoue, K. Masatsugu, Y. Fukunaga, N. Sawada, K. Tojo, Y. Saito, T. Hosoya, K. Nakao

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To address endothelial function on vascular walls exposed to oxidative stress, we investigated the effect of oxidative stress on the secretion of endothelin-1 (ET-1) from cultured bovine carotid artery endothelial cells (BAECs). Concentrations of ET-1 in the media were measured by a specific radioimmunoassay and ET-1 mRNA expression was estimated by Northern blot analysis. Treatment of BAECs with 0.5-2.0 mM H2O2 for 3 h suppressed both ET-1 secretion and ET-1 mRNA expression in a dose-dependent manner compared to control. Attenuation of ET-1 mRNA expression by H2O2 was revealed to take place at the transcriptional level. The addition of NG-nitro-L-arginine-methyl ester (L-NAME) 10 μm, a specific nitric oxide synthase inhibitor, had no effect on H2O2-induced suppression of ET-1 mRNA expression. Suppression of ET secretion under oxidative stress observed in the present study is proposed to be a compensatory mechanism of endothelial cells to inhibit vasoconstriction and proliferation during oxidative stress.

Original languageEnglish
JournalJournal of Cardiovascular Pharmacology
Volume31
Issue numberSUPPL. 1
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Endothelins
Endothelin-1
Oxidative Stress
Messenger RNA
Endothelial Cells
NG-Nitroarginine Methyl Ester
Carotid Arteries
Vasoconstriction
Nitric Oxide Synthase
Northern Blotting
Radioimmunoassay
Blood Vessels

Keywords

  • Endothelial cells
  • Endothelin
  • Hydrogen peroxide
  • L-NAME
  • Oxidative stress

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

Cite this

Saito, T., Itoh, H., Chun, T., Igaki, T., Mori, Y., Yamashita, J., ... Nakao, K. (1998). Oxidative stress suppresses the endothelial secretion of endothelin. Journal of Cardiovascular Pharmacology, 31(SUPPL. 1).

Oxidative stress suppresses the endothelial secretion of endothelin. / Saito, T.; Itoh, Hiroshi; Chun, T.; Igaki, T.; Mori, Y.; Yamashita, J.; Doi, K.; Tanaka, T.; Inoue, M.; Masatsugu, K.; Fukunaga, Y.; Sawada, N.; Tojo, K.; Saito, Y.; Hosoya, T.; Nakao, K.

In: Journal of Cardiovascular Pharmacology, Vol. 31, No. SUPPL. 1, 1998.

Research output: Contribution to journalArticle

Saito, T, Itoh, H, Chun, T, Igaki, T, Mori, Y, Yamashita, J, Doi, K, Tanaka, T, Inoue, M, Masatsugu, K, Fukunaga, Y, Sawada, N, Tojo, K, Saito, Y, Hosoya, T & Nakao, K 1998, 'Oxidative stress suppresses the endothelial secretion of endothelin', Journal of Cardiovascular Pharmacology, vol. 31, no. SUPPL. 1.
Saito, T. ; Itoh, Hiroshi ; Chun, T. ; Igaki, T. ; Mori, Y. ; Yamashita, J. ; Doi, K. ; Tanaka, T. ; Inoue, M. ; Masatsugu, K. ; Fukunaga, Y. ; Sawada, N. ; Tojo, K. ; Saito, Y. ; Hosoya, T. ; Nakao, K. / Oxidative stress suppresses the endothelial secretion of endothelin. In: Journal of Cardiovascular Pharmacology. 1998 ; Vol. 31, No. SUPPL. 1.
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