Oxiradical-dependent photoemission induced by a phacoemulsification probe

Shigeto Shimmura, Kazuo Tsubota, Y. Oguchi, D. Fukumura, M. Suematsu, M. Tsuchiya

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Oxygen free radical formation by conventional phacoemulsification devices has been postulated as a possible mechanism of corneal endothelial damage during surgery. To test this hypothesis, phacoemulsification probe-induced free radical production was visualized using a single photon-counting camera and an O2--sensitive luciferin derivative, 2-methyl-6-[p-methoxyphenyl-3,7- dihydroimidazo [1,2-a]pyrazin-3-one (MCLA), which allows the visualization of spatial and temporal alterations in free radical production. Within 1 min after starting ultrasound emission, MCLA-dependent chemiluminescence was increased significantly, the intensity of which was maximal at the tip of the probe and tapered along a gradient toward distal portions. The chemiluminescence was suppressed significantly by adding either superoxide dismutase (300 U/ml) or sodium azide (20 mmol/l). By adding deuterium to the medium, MCLA-dependent chemiluminescence significantly increased, suggesting the involvement of singlet oxygen in the reaction.

Original languageEnglish
Pages (from-to)2904-2907
Number of pages4
JournalInvestigative Ophthalmology and Visual Science
Volume33
Issue number10
Publication statusPublished - 1992

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Phacoemulsification
Luminescence
Free Radicals
Sodium Azide
Singlet Oxygen
Deuterium
Photons
Superoxide Dismutase
Reactive Oxygen Species
Equipment and Supplies
2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo(1,2-alpha)pyrazin-3-one

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Oxiradical-dependent photoemission induced by a phacoemulsification probe. / Shimmura, Shigeto; Tsubota, Kazuo; Oguchi, Y.; Fukumura, D.; Suematsu, M.; Tsuchiya, M.

In: Investigative Ophthalmology and Visual Science, Vol. 33, No. 10, 1992, p. 2904-2907.

Research output: Contribution to journalArticle

Shimmura, S, Tsubota, K, Oguchi, Y, Fukumura, D, Suematsu, M & Tsuchiya, M 1992, 'Oxiradical-dependent photoemission induced by a phacoemulsification probe', Investigative Ophthalmology and Visual Science, vol. 33, no. 10, pp. 2904-2907.
Shimmura, Shigeto ; Tsubota, Kazuo ; Oguchi, Y. ; Fukumura, D. ; Suematsu, M. ; Tsuchiya, M. / Oxiradical-dependent photoemission induced by a phacoemulsification probe. In: Investigative Ophthalmology and Visual Science. 1992 ; Vol. 33, No. 10. pp. 2904-2907.
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