P-selectin reexpresses and supports leukocyte adherence on the endothelial cell surfaces by repetitive stimulations with thrombin

H. Kameda, I. Marita, S. Murota, M. Handa, L. Haria, L. Yoshida, T. Mimgori, I. Ikeda

Research output: Contribution to journalArticle

Abstract

P-selectin is a cell adhesion molecule, which is located on the granele membrane of Weibel-Palade bodies in resting endothelial cells and expressed on the cell surfaces during celiutar activation/ foil owed by internal ization. So we investigated whether P-selectin reexpresses with subsequent cellular activation. Indirect Immunofluorescence and analysis with confocal laser cytometer (ACAS 570) led us to measure the celfular P-selectin content and surface expression of it. The surface expression of P-selectin is increased to the maximum within 2 minutes of thrombin (l U/ml) stimulation and declined to basal level after 180 minutes. Rechallenge with thrombin induced rapid reexpression of P-selectin on the endothelial surfaces. Cellular P-selectin content showed little decline throughout those courses. Moreover, reexpressed P-selectin remained the function for supporting the adherence of promyelocytic cell line HL60 cells. These results demonstrated the recycling mechanism of P-selectin, which is in contrast to cytokine-inducible adhesion molecules such as E-selectin, and provided the possibility that P-selectin might be a candidate even in chronic inflammatory diseases.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number6
Publication statusPublished - 1996

Fingerprint

P-Selectin
thrombin
Endothelial cells
Thrombin
endothelial cells
leukocytes
Leukocytes
Endothelial Cells
foil
cell adhesion
fluorescent antibody technique
recycling
lasers
adhesion
cytokines
cell lines
cells
Weibel-Palade Bodies
Chemical activation
E-Selectin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Kameda, H., Marita, I., Murota, S., Handa, M., Haria, L., Yoshida, L., ... Ikeda, I. (1996). P-selectin reexpresses and supports leukocyte adherence on the endothelial cell surfaces by repetitive stimulations with thrombin. FASEB Journal, 10(6).

P-selectin reexpresses and supports leukocyte adherence on the endothelial cell surfaces by repetitive stimulations with thrombin. / Kameda, H.; Marita, I.; Murota, S.; Handa, M.; Haria, L.; Yoshida, L.; Mimgori, T.; Ikeda, I.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

Kameda, H, Marita, I, Murota, S, Handa, M, Haria, L, Yoshida, L, Mimgori, T & Ikeda, I 1996, 'P-selectin reexpresses and supports leukocyte adherence on the endothelial cell surfaces by repetitive stimulations with thrombin', FASEB Journal, vol. 10, no. 6.
Kameda, H. ; Marita, I. ; Murota, S. ; Handa, M. ; Haria, L. ; Yoshida, L. ; Mimgori, T. ; Ikeda, I. / P-selectin reexpresses and supports leukocyte adherence on the endothelial cell surfaces by repetitive stimulations with thrombin. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
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AU - Marita, I.

AU - Murota, S.

AU - Handa, M.

AU - Haria, L.

AU - Yoshida, L.

AU - Mimgori, T.

AU - Ikeda, I.

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