P-selectin reexpresses and supports leukocyte adherence on the endothelial cell surfaces by repetitive stimulations with thrombin

H. Kameda, I. Marita, S. Murota, M. Handa, L. Haria, L. Yoshida, T. Mimgori, I. Ikeda

Research output: Contribution to journalArticle

Abstract

P-selectin is a cell adhesion molecule, which is located on the granele membrane of Weibel-Palade bodies in resting endothelial cells and expressed on the cell surfaces during celiutar activation/ foil owed by internal ization. So we investigated whether P-selectin reexpresses with subsequent cellular activation. Indirect Immunofluorescence and analysis with confocal laser cytometer (ACAS 570) led us to measure the celfular P-selectin content and surface expression of it. The surface expression of P-selectin is increased to the maximum within 2 minutes of thrombin (l U/ml) stimulation and declined to basal level after 180 minutes. Rechallenge with thrombin induced rapid reexpression of P-selectin on the endothelial surfaces. Cellular P-selectin content showed little decline throughout those courses. Moreover, reexpressed P-selectin remained the function for supporting the adherence of promyelocytic cell line HL60 cells. These results demonstrated the recycling mechanism of P-selectin, which is in contrast to cytokine-inducible adhesion molecules such as E-selectin, and provided the possibility that P-selectin might be a candidate even in chronic inflammatory diseases.

Original languageEnglish
Pages (from-to)A1199
JournalFASEB Journal
Volume10
Issue number6
Publication statusPublished - 1996 Dec 1

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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    Kameda, H., Marita, I., Murota, S., Handa, M., Haria, L., Yoshida, L., Mimgori, T., & Ikeda, I. (1996). P-selectin reexpresses and supports leukocyte adherence on the endothelial cell surfaces by repetitive stimulations with thrombin. FASEB Journal, 10(6), A1199.