TY - JOUR
T1 - P38MAPK and Rho-dependent kinase are involved in anoikis induced by anicequol or 25-hydroxycholesterol in DLD-1 colon cancer cells
AU - Tanaka, Arowu R.
AU - Noguchi, Kohji
AU - Fukazawa, Hidesuke
AU - Igarashi, Yasuhiro
AU - Arai, Hiroyuki
AU - Uehara, Yoshimasa
N1 - Funding Information:
We gratefully acknowledge helpful discussions of Drs. Naoyuki Nishiya and Kayoko Tsuda. Financial support for this study was provided by Ministry of Education, Culture, Sports, Science and Technology of Japan .
PY - 2013/1/25
Y1 - 2013/1/25
N2 - Anchorage-independent growth is evidence of the malignant transformation of cells. We previously reported the characterization of anicequol, a novel inhibitor of the anchorage-independent growth of tumor cells, and here we show that the effects of 25-hydroxycholesterol (25-HC) on colon cancer cells were very similar to those of anicequol. By analyzing the effects of inhibitors and performing RNA interference experiments, we found that p38 mitogen-activated protein kinase (p38MAPK) was involved in anicequol- and 25-HC-induced anoikis in DLD-1 cells. In addition, Rho-associated, coiled-coil containing protein kinase (ROCK) was also associated with anoikis induced by anicequol or 25-HC. Taken together, our findings suggest that activation of the p38MAPK and ROCK pathways might provide a new therapeutic strategy against cancer, and raise the possibility that tumor metastasis is influenced by 25-HC under physiological conditions.
AB - Anchorage-independent growth is evidence of the malignant transformation of cells. We previously reported the characterization of anicequol, a novel inhibitor of the anchorage-independent growth of tumor cells, and here we show that the effects of 25-hydroxycholesterol (25-HC) on colon cancer cells were very similar to those of anicequol. By analyzing the effects of inhibitors and performing RNA interference experiments, we found that p38 mitogen-activated protein kinase (p38MAPK) was involved in anicequol- and 25-HC-induced anoikis in DLD-1 cells. In addition, Rho-associated, coiled-coil containing protein kinase (ROCK) was also associated with anoikis induced by anicequol or 25-HC. Taken together, our findings suggest that activation of the p38MAPK and ROCK pathways might provide a new therapeutic strategy against cancer, and raise the possibility that tumor metastasis is influenced by 25-HC under physiological conditions.
KW - 25-Hydroxycholesterol
KW - Anicequol
KW - Anoikis
KW - Coiled-coil containing protein kinase (ROCK)
KW - P38MAPK
KW - Rho-associated
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UR - http://www.scopus.com/inward/citedby.url?scp=84872868818&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2012.12.067
DO - 10.1016/j.bbrc.2012.12.067
M3 - Article
C2 - 23266613
AN - SCOPUS:84872868818
SN - 0006-291X
VL - 430
SP - 1240
EP - 1245
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -
