P38MAPK and Rho-dependent kinase are involved in anoikis induced by anicequol or 25-hydroxycholesterol in DLD-1 colon cancer cells

Arowu R. Tanaka, Kohji Noguchi, Hidesuke Fukazawa, Yasuhiro Igarashi, Hiroyuki Arai, Yoshimasa Uehara

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Anchorage-independent growth is evidence of the malignant transformation of cells. We previously reported the characterization of anicequol, a novel inhibitor of the anchorage-independent growth of tumor cells, and here we show that the effects of 25-hydroxycholesterol (25-HC) on colon cancer cells were very similar to those of anicequol. By analyzing the effects of inhibitors and performing RNA interference experiments, we found that p38 mitogen-activated protein kinase (p38MAPK) was involved in anicequol- and 25-HC-induced anoikis in DLD-1 cells. In addition, Rho-associated, coiled-coil containing protein kinase (ROCK) was also associated with anoikis induced by anicequol or 25-HC. Taken together, our findings suggest that activation of the p38MAPK and ROCK pathways might provide a new therapeutic strategy against cancer, and raise the possibility that tumor metastasis is influenced by 25-HC under physiological conditions.

Original languageEnglish
Pages (from-to)1240-1245
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume430
Issue number4
DOIs
Publication statusPublished - 2013 Jan 25

Fingerprint

Anoikis
rho-Associated Kinases
Colonic Neoplasms
Phosphotransferases
Cells
p38 Mitogen-Activated Protein Kinases
Tumors
Neoplasms
Growth
RNA Interference
Chemical activation
RNA
Neoplasm Metastasis
25-hydroxycholesterol
anicequol
Experiments

Keywords

  • 25-Hydroxycholesterol
  • Anicequol
  • Anoikis
  • Coiled-coil containing protein kinase (ROCK)
  • P38MAPK
  • Rho-associated

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

P38MAPK and Rho-dependent kinase are involved in anoikis induced by anicequol or 25-hydroxycholesterol in DLD-1 colon cancer cells. / Tanaka, Arowu R.; Noguchi, Kohji; Fukazawa, Hidesuke; Igarashi, Yasuhiro; Arai, Hiroyuki; Uehara, Yoshimasa.

In: Biochemical and Biophysical Research Communications, Vol. 430, No. 4, 25.01.2013, p. 1240-1245.

Research output: Contribution to journalArticle

Tanaka, Arowu R. ; Noguchi, Kohji ; Fukazawa, Hidesuke ; Igarashi, Yasuhiro ; Arai, Hiroyuki ; Uehara, Yoshimasa. / P38MAPK and Rho-dependent kinase are involved in anoikis induced by anicequol or 25-hydroxycholesterol in DLD-1 colon cancer cells. In: Biochemical and Biophysical Research Communications. 2013 ; Vol. 430, No. 4. pp. 1240-1245.
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