P38MAPK and Rho-dependent kinase are involved in anoikis induced by anicequol or 25-hydroxycholesterol in DLD-1 colon cancer cells

Arowu R. Tanaka, Kohji Noguchi, Hidesuke Fukazawa, Yasuhiro Igarashi, Hiroyuki Arai, Yoshimasa Uehara

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Anchorage-independent growth is evidence of the malignant transformation of cells. We previously reported the characterization of anicequol, a novel inhibitor of the anchorage-independent growth of tumor cells, and here we show that the effects of 25-hydroxycholesterol (25-HC) on colon cancer cells were very similar to those of anicequol. By analyzing the effects of inhibitors and performing RNA interference experiments, we found that p38 mitogen-activated protein kinase (p38MAPK) was involved in anicequol- and 25-HC-induced anoikis in DLD-1 cells. In addition, Rho-associated, coiled-coil containing protein kinase (ROCK) was also associated with anoikis induced by anicequol or 25-HC. Taken together, our findings suggest that activation of the p38MAPK and ROCK pathways might provide a new therapeutic strategy against cancer, and raise the possibility that tumor metastasis is influenced by 25-HC under physiological conditions.

Original languageEnglish
Pages (from-to)1240-1245
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume430
Issue number4
DOIs
Publication statusPublished - 2013 Jan 25

Keywords

  • 25-Hydroxycholesterol
  • Anicequol
  • Anoikis
  • Coiled-coil containing protein kinase (ROCK)
  • P38MAPK
  • Rho-associated

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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