Parallel recognition of single-stranded DNA using a biological nanopore array

Y. Tsuji, R. Kawano, T. Osaki, H. Sasaki, Norihisa Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper describes simultaneous detections of a single DNA molecule using a biological nanopore in an array device (Figure 1a). In the previous report, we have successfully reconstituted α-hemolysin αHL) in bilayer lipid membranes (BLMs) using droplets contacting method[1]. In this research, we attempted to detect single-stranded DNA (ssDNA) translocation events through the protein channel. As a result, we succeeded in observing ssDNA translocation events in 15ch simultaneously. We believe that this method can be applied to an automated and high-throughput DNA analysis.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1284-1286
Number of pages3
Volume2
Publication statusPublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Fingerprint

Nanopores
DNA
Lipid bilayers
Throughput
Proteins
Molecules

Keywords

  • α-Hemolysin αHL)
  • Bilayer lipid membranes (BLMs)
  • Droplets contacting method
  • Single-stranded DNA (ssDNA)

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Tsuji, Y., Kawano, R., Osaki, T., Sasaki, H., Miki, N., & Takeuchi, S. (2011). Parallel recognition of single-stranded DNA using a biological nanopore array. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 2, pp. 1284-1286)

Parallel recognition of single-stranded DNA using a biological nanopore array. / Tsuji, Y.; Kawano, R.; Osaki, T.; Sasaki, H.; Miki, Norihisa; Takeuchi, S.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2 2011. p. 1284-1286.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tsuji, Y, Kawano, R, Osaki, T, Sasaki, H, Miki, N & Takeuchi, S 2011, Parallel recognition of single-stranded DNA using a biological nanopore array. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 2, pp. 1284-1286, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 11/10/2.
Tsuji Y, Kawano R, Osaki T, Sasaki H, Miki N, Takeuchi S. Parallel recognition of single-stranded DNA using a biological nanopore array. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2. 2011. p. 1284-1286
Tsuji, Y. ; Kawano, R. ; Osaki, T. ; Sasaki, H. ; Miki, Norihisa ; Takeuchi, S. / Parallel recognition of single-stranded DNA using a biological nanopore array. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2 2011. pp. 1284-1286
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