Parallel recognition of single-stranded DNA using a biological nanopore array

Y. Tsuji, R. Kawano, T. Osaki, H. Sasaki, N. Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper describes simultaneous detections of a single DNA molecule using a biological nanopore in an array device (Figure 1a). In the previous report, we have successfully reconstituted α-hemolysin αHL) in bilayer lipid membranes (BLMs) using droplets contacting method[1]. In this research, we attempted to detect single-stranded DNA (ssDNA) translocation events through the protein channel. As a result, we succeeded in observing ssDNA translocation events in 15ch simultaneously. We believe that this method can be applied to an automated and high-throughput DNA analysis.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1284-1286
Number of pages3
Publication statusPublished - 2011 Dec 1
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

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Keywords

  • Bilayer lipid membranes (BLMs)
  • Droplets contacting method
  • Single-stranded DNA (ssDNA)
  • α-Hemolysin αHL)

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Tsuji, Y., Kawano, R., Osaki, T., Sasaki, H., Miki, N., & Takeuchi, S. (2011). Parallel recognition of single-stranded DNA using a biological nanopore array. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1284-1286). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).