Parametric imaging of the chick embryonic cardiovascular system

A novel functional measure

Kenjiro Kosaki, Hidekazu Suzuki, Geert W. Schmid-Schönbein, Thomas R. Nelson, Kenneth Lyons Jones

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Morphogenesis of the cardiovascular system is likely linked to its functional development. This report presents an approach to functional assessment of early cardiovascular development using parametric imaging based on videodensitometry. Trypan blue was injected into the sinus venosus of chick embryos at stage 20 (3.5 d of incubation). Images were recorded on videotape, digitized, and analyzed by a microcomputer. A sampling window was placed over the entire image, and a densogram (time density curve) for each pixel in the window was obtained. Parameters extracted from the densogram and its derivative were plotted in form of: 1) maximal image, 2) peak derivative image, and 3) time to peak derivative image. This approach revealed isochronal lines that divide the aortic arch and dorsal aorta into several segments. Regional flow velocity at these segments was estimated by dividing the distance between isochronal lines by the time interval. Flow velocity at the mid-systolic phase at the dorsal aorta and at the fourth aortic arch was 35.9 and 45.0 mm/s, respectively. Shear rate at the vessel wall was estimated to be 2.7 times larger at the fourth aortic arch than at the dorsal aorta. The extensive remodeling experienced by the aortic arch system compared with the dorsal aorta could be related to increased shear rate on the walls of the aortic arches.

Original languageEnglish
Pages (from-to)451-456
Number of pages6
JournalPediatric Research
Volume41
Issue number4 I
Publication statusPublished - 1997 Apr
Externally publishedYes

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Cardiovascular System
Thoracic Aorta
Aorta
Videotape Recording
Trypan Blue
Microcomputers
Chick Embryo
Morphogenesis

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Kosaki, K., Suzuki, H., Schmid-Schönbein, G. W., Nelson, T. R., & Jones, K. L. (1997). Parametric imaging of the chick embryonic cardiovascular system: A novel functional measure. Pediatric Research, 41(4 I), 451-456.

Parametric imaging of the chick embryonic cardiovascular system : A novel functional measure. / Kosaki, Kenjiro; Suzuki, Hidekazu; Schmid-Schönbein, Geert W.; Nelson, Thomas R.; Jones, Kenneth Lyons.

In: Pediatric Research, Vol. 41, No. 4 I, 04.1997, p. 451-456.

Research output: Contribution to journalArticle

Kosaki, K, Suzuki, H, Schmid-Schönbein, GW, Nelson, TR & Jones, KL 1997, 'Parametric imaging of the chick embryonic cardiovascular system: A novel functional measure', Pediatric Research, vol. 41, no. 4 I, pp. 451-456.
Kosaki K, Suzuki H, Schmid-Schönbein GW, Nelson TR, Jones KL. Parametric imaging of the chick embryonic cardiovascular system: A novel functional measure. Pediatric Research. 1997 Apr;41(4 I):451-456.
Kosaki, Kenjiro ; Suzuki, Hidekazu ; Schmid-Schönbein, Geert W. ; Nelson, Thomas R. ; Jones, Kenneth Lyons. / Parametric imaging of the chick embryonic cardiovascular system : A novel functional measure. In: Pediatric Research. 1997 ; Vol. 41, No. 4 I. pp. 451-456.
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