Parathyroid hormone activation of the 25-hydroxyvitamin D3-1α- hydroxylase gene promoter

H. L. Brenza, C. Kimmel-Jehan, F. Jehan, T. Shinki, Shu Wakino, H. Anazawa, T. Suda, H. F. DeLuca

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

The DNA flanking the 5' sequence of the mouse 1α-hydroxylase gene has been cloned and sequenced. A TATA box has been located at -30 bp and aCCAAT box has been located at -79 bp. The gene's promoter activity has been demonstrated by using a luciferase reporter gene construct transfected into a modified pig kidney cell line, AOK-B50. Parathyroid hormone stimulates this promoter-directed synthesis of luciferase by 17-fold, whereas forskolin stimulates it by 3-fold. The action of parathyroid hormone is concentration- dependent. 1,25-Dihydroxyvitamin D3 does not suppress basal promoter activity and marginally suppresses parathyroid hormone-driven luciferase reporter activity. The promoter has three potential cAMP-responsive element sites, and two perfect and one imperfect AP-1 sites, while no DR-3 was detected. These results indicate that parathyroid hormone stimulates 25- hydroxyvitamin D3-1α-hydroxylase by acting on the promoter of the 1α- hydroxylase gene.

Original languageEnglish
Pages (from-to)1387-1391
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number4
DOIs
Publication statusPublished - 1998 Feb 17

Fingerprint

Calcifediol
Mixed Function Oxygenases
Parathyroid Hormone
Luciferases
Genes
TATA Box
Calcitriol
5' Flanking Region
Transcription Factor AP-1
Colforsin
Reporter Genes
Swine
Kidney
Cell Line
DNA

Keywords

  • Bone
  • Calcium
  • Cyclic AMP
  • Kidney
  • Vitamin D

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Parathyroid hormone activation of the 25-hydroxyvitamin D3-1α- hydroxylase gene promoter. / Brenza, H. L.; Kimmel-Jehan, C.; Jehan, F.; Shinki, T.; Wakino, Shu; Anazawa, H.; Suda, T.; DeLuca, H. F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 4, 17.02.1998, p. 1387-1391.

Research output: Contribution to journalArticle

Brenza, H. L. ; Kimmel-Jehan, C. ; Jehan, F. ; Shinki, T. ; Wakino, Shu ; Anazawa, H. ; Suda, T. ; DeLuca, H. F. / Parathyroid hormone activation of the 25-hydroxyvitamin D3-1α- hydroxylase gene promoter. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 4. pp. 1387-1391.
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AU - Wakino, Shu

AU - Anazawa, H.

AU - Suda, T.

AU - DeLuca, H. F.

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