Parathyroid hormone-related protein as a common target molecule in specific immunotherapy for a wide variety of tumor types

Yoshimi Arima, Satoko Matsueda, Hirohisa Yano, Mamoru Harada, Kyogo Itoh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Parathyroid hormone-related protein (PTH-rP) has been considered to be responsible for malignancy-associated hypercalcemia and is thought to participate in pathological changes in bone metastases of cancer. In this study, we determined whether or not PTH-rP could be a common target molecule in specific immunotherapy for patients with a wide variety of tumor types. Various types of tumor cell lines were examined for PTH-rP expression at the mRNA and protein levels by RT-PCR, flow cytometry, and immunocytochemistry. We also determined whether or not cancer-reactive cytotoxic T lymphocytes (CTLs) could be induced from the peripheral blood mononuclear cells (PBMCs) of HLA-A24 + patients with gastric, colon, renal, or cervical cancer by in vitro stimulation with two PTH-rP peptides. As a result, PTH-rP mRNA was expressed in the majority of gastric, breast, lung, colon, cervical, and renal cancer cell lines. Expression of the protein was confirmed by both flow cytometry and immunocytochemistry. Furthermore, PTH-rP peptide-specific and cancerreactive CTLs were successfully generated from the PBMCs of HLA-A24+ patients with different tumor types using in vitro stimulation with either the PTH-rP102-111 or PTH-rP, 110-119 peptide. These findings indicate that PTH-rP could be a common target molecule in specific immunotherapy for patients with a wide variety of tumor types, particularly bone metastases.

Original languageEnglish
Pages (from-to)981-988
Number of pages8
JournalInternational journal of oncology
Volume27
Issue number4
Publication statusPublished - 2005 Oct 1
Externally publishedYes

Keywords

  • Cytotoxic T lymphocytes
  • HLA-A24
  • Immunotherapy
  • Parathyroid hormone-related protein
  • Peptide

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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