Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent phosphorylation

Masahiro Iguchi, Yuki Kujuro, Kei Okatsu, Fumika Koyano, Hidetaka Kosako, Mayumi Kimura, Norihiro Suzuki, Shinichiro Uchiyama, Keiji Tanaka, Noriyuki Matsuda

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Background: Parkin is a ubiquitin ligase activated by a decrease in the mitochondrial membrane potential (Δ Φm). However, details regarding its mechanism remain limited. Results: PINK1-dependent phosphorylation of Parkin at Ser-65 following dissipation of Δ Φm triggers ubiquitin-ester transfer by the RING2 domain of Parkin to Cys-431. Conclusion: Parkin catalyzes trans- (ubiquitin-thioester)ification upon PINK1-dependent phosphorylation. Significance: The molecular process of Parkin-catalyzed ubiquitylation has been determined.

Original languageEnglish
Pages (from-to)22019-22032
Number of pages14
JournalJournal of Biological Chemistry
Volume288
Issue number30
DOIs
Publication statusPublished - 2013 Jul 26

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Phosphorylation
Ubiquitin
Esters
Mitochondrial Membrane Potential
Ubiquitination
Ligases
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Iguchi, M., Kujuro, Y., Okatsu, K., Koyano, F., Kosako, H., Kimura, M., ... Matsuda, N. (2013). Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent phosphorylation. Journal of Biological Chemistry, 288(30), 22019-22032. https://doi.org/10.1074/jbc.M113.467530

Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent phosphorylation. / Iguchi, Masahiro; Kujuro, Yuki; Okatsu, Kei; Koyano, Fumika; Kosako, Hidetaka; Kimura, Mayumi; Suzuki, Norihiro; Uchiyama, Shinichiro; Tanaka, Keiji; Matsuda, Noriyuki.

In: Journal of Biological Chemistry, Vol. 288, No. 30, 26.07.2013, p. 22019-22032.

Research output: Contribution to journalArticle

Iguchi, M, Kujuro, Y, Okatsu, K, Koyano, F, Kosako, H, Kimura, M, Suzuki, N, Uchiyama, S, Tanaka, K & Matsuda, N 2013, 'Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent phosphorylation', Journal of Biological Chemistry, vol. 288, no. 30, pp. 22019-22032. https://doi.org/10.1074/jbc.M113.467530
Iguchi, Masahiro ; Kujuro, Yuki ; Okatsu, Kei ; Koyano, Fumika ; Kosako, Hidetaka ; Kimura, Mayumi ; Suzuki, Norihiro ; Uchiyama, Shinichiro ; Tanaka, Keiji ; Matsuda, Noriyuki. / Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent phosphorylation. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 30. pp. 22019-22032.
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AU - Kimura, Mayumi

AU - Suzuki, Norihiro

AU - Uchiyama, Shinichiro

AU - Tanaka, Keiji

AU - Matsuda, Noriyuki

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