PARP-1 regulates expression of TGF-β receptors in T cells

Pin Zhang, Hiroko Nakatsukasa, Eric Tu, Shimpei Kasagi, Kairong Cui, Masaki Ishikawa, Joanne E. Konkel, Takashi Maruyama, Gang Wei, Brittany Abbatiello, Zhao Qi Wang, Keji Zhao, Wan Jun Chen

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Transforming growth factor-b (TGF-b) receptors (TbRs) are essential components for TGF-b signal transduction in T cells, yet the mechanisms by which the receptors are regulated remain poorly understood. We show here that Poly(ADP-ribose) polymerase-1 (PARP-1) regulates TGF-b receptor I (TbRI) and II (TbRII) expression in CD4+ T cells and subsequently affects Smad2/3-mediated TGF-b signal transduction. Inhibition of PARP-1 led to the upregulation of both TbRI and TbRII, yet the underlying molecular mechanisms were distinct. PARP-1 selectively bound to the promoter of TbRII, whereas the enzymatic activity of PARP-1 was responsible for the inhibition of TbRI expression. Importantly, inhibition of PARP-1 also enhanced expression of TbRs in human CD4+ T cells. Thus, PARP-1 regulates TbR expression and TGF-b signaling in T cells.

Original languageEnglish
Pages (from-to)2224-2232
Number of pages9
JournalBlood
Volume122
Issue number13
DOIs
Publication statusPublished - 2013 Jan 1
Externally publishedYes

Fingerprint

T-cells
Poly(ADP-ribose) Polymerases
T-Cell Antigen Receptor
Transforming Growth Factors
T-Lymphocytes
Signal transduction
Growth Factor Receptors
Signal Transduction
Poly (ADP-Ribose) Polymerase-1
Up-Regulation

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Zhang, P., Nakatsukasa, H., Tu, E., Kasagi, S., Cui, K., Ishikawa, M., ... Chen, W. J. (2013). PARP-1 regulates expression of TGF-β receptors in T cells. Blood, 122(13), 2224-2232. https://doi.org/10.1182/blood-2013-05-503250

PARP-1 regulates expression of TGF-β receptors in T cells. / Zhang, Pin; Nakatsukasa, Hiroko; Tu, Eric; Kasagi, Shimpei; Cui, Kairong; Ishikawa, Masaki; Konkel, Joanne E.; Maruyama, Takashi; Wei, Gang; Abbatiello, Brittany; Wang, Zhao Qi; Zhao, Keji; Chen, Wan Jun.

In: Blood, Vol. 122, No. 13, 01.01.2013, p. 2224-2232.

Research output: Contribution to journalArticle

Zhang, P, Nakatsukasa, H, Tu, E, Kasagi, S, Cui, K, Ishikawa, M, Konkel, JE, Maruyama, T, Wei, G, Abbatiello, B, Wang, ZQ, Zhao, K & Chen, WJ 2013, 'PARP-1 regulates expression of TGF-β receptors in T cells', Blood, vol. 122, no. 13, pp. 2224-2232. https://doi.org/10.1182/blood-2013-05-503250
Zhang P, Nakatsukasa H, Tu E, Kasagi S, Cui K, Ishikawa M et al. PARP-1 regulates expression of TGF-β receptors in T cells. Blood. 2013 Jan 1;122(13):2224-2232. https://doi.org/10.1182/blood-2013-05-503250
Zhang, Pin ; Nakatsukasa, Hiroko ; Tu, Eric ; Kasagi, Shimpei ; Cui, Kairong ; Ishikawa, Masaki ; Konkel, Joanne E. ; Maruyama, Takashi ; Wei, Gang ; Abbatiello, Brittany ; Wang, Zhao Qi ; Zhao, Keji ; Chen, Wan Jun. / PARP-1 regulates expression of TGF-β receptors in T cells. In: Blood. 2013 ; Vol. 122, No. 13. pp. 2224-2232.
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