Partial nuclear localization of a bovine phosphoprotein, BCNT, that includes a region derived from a LINE repetitive sequence in Ruminantia

Shintaro Iwashita, Takahiro Nobukuni, Shoji Tanaka, Mariko Kobayashi, Toshihiko Iwanaga, Hidetoshi B. Tamate, Toru Masui, Ichiro Takahashi, Katsuyuki Hashimoto

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

BCNT, named after Bucentaur, is a protein that contains a 324-amino-acid region derived from part of a long interspersed DNA sequence element (LINE) in Ruminantia. However, the unique portion is completely missing in human and mouse BCNTs. Since no significant information on their function has been obtained by homology search, we at first examined cellular localization and biochemical characteristics of bovine BCNT to get a hint on its function. Subcellular fractionation and immunohistochemical analyses using a normal bovine epithelial cell line and bovine brain revealed that a significant amount of bovine BCNT is localized in the nuclei, while the major portion is present in the cytosol. Furthermore, it was shown that bovine BCNT is a phosphoprotein and that both bovine and human BCNTs are phosphorylated by casein kinase II in vitro. These results show that BCNTs consist of a unique family, probably a substrate of casein kinase II, which may contribute further to the understanding of gene evolution. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)408-416
Number of pages9
JournalBiochimica et Biophysica Acta - General Subjects
Volume1427
Issue number3
DOIs
Publication statusPublished - 1999 May 24
Externally publishedYes

Fingerprint

Casein Kinase II
Nucleic Acid Repetitive Sequences
Phosphoproteins
Ruminants
Long Interspersed Nucleotide Elements
Fractionation
Brain
Genes
Amino Acids
Substrates
Proteins
Cytosol
Epithelial Cells
Cell Line

Keywords

  • LINE insertion
  • Nuclear localization
  • Phosphoprotein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Partial nuclear localization of a bovine phosphoprotein, BCNT, that includes a region derived from a LINE repetitive sequence in Ruminantia. / Iwashita, Shintaro; Nobukuni, Takahiro; Tanaka, Shoji; Kobayashi, Mariko; Iwanaga, Toshihiko; Tamate, Hidetoshi B.; Masui, Toru; Takahashi, Ichiro; Hashimoto, Katsuyuki.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1427, No. 3, 24.05.1999, p. 408-416.

Research output: Contribution to journalArticle

Iwashita, S, Nobukuni, T, Tanaka, S, Kobayashi, M, Iwanaga, T, Tamate, HB, Masui, T, Takahashi, I & Hashimoto, K 1999, 'Partial nuclear localization of a bovine phosphoprotein, BCNT, that includes a region derived from a LINE repetitive sequence in Ruminantia', Biochimica et Biophysica Acta - General Subjects, vol. 1427, no. 3, pp. 408-416. https://doi.org/10.1016/S0304-4165(99)00049-5
Iwashita, Shintaro ; Nobukuni, Takahiro ; Tanaka, Shoji ; Kobayashi, Mariko ; Iwanaga, Toshihiko ; Tamate, Hidetoshi B. ; Masui, Toru ; Takahashi, Ichiro ; Hashimoto, Katsuyuki. / Partial nuclear localization of a bovine phosphoprotein, BCNT, that includes a region derived from a LINE repetitive sequence in Ruminantia. In: Biochimica et Biophysica Acta - General Subjects. 1999 ; Vol. 1427, No. 3. pp. 408-416.
@article{d27625e83c9e4a1bb02f6c093fb216fa,
title = "Partial nuclear localization of a bovine phosphoprotein, BCNT, that includes a region derived from a LINE repetitive sequence in Ruminantia",
abstract = "BCNT, named after Bucentaur, is a protein that contains a 324-amino-acid region derived from part of a long interspersed DNA sequence element (LINE) in Ruminantia. However, the unique portion is completely missing in human and mouse BCNTs. Since no significant information on their function has been obtained by homology search, we at first examined cellular localization and biochemical characteristics of bovine BCNT to get a hint on its function. Subcellular fractionation and immunohistochemical analyses using a normal bovine epithelial cell line and bovine brain revealed that a significant amount of bovine BCNT is localized in the nuclei, while the major portion is present in the cytosol. Furthermore, it was shown that bovine BCNT is a phosphoprotein and that both bovine and human BCNTs are phosphorylated by casein kinase II in vitro. These results show that BCNTs consist of a unique family, probably a substrate of casein kinase II, which may contribute further to the understanding of gene evolution. Copyright (C) 1999 Elsevier Science B.V.",
keywords = "LINE insertion, Nuclear localization, Phosphoprotein",
author = "Shintaro Iwashita and Takahiro Nobukuni and Shoji Tanaka and Mariko Kobayashi and Toshihiko Iwanaga and Tamate, {Hidetoshi B.} and Toru Masui and Ichiro Takahashi and Katsuyuki Hashimoto",
year = "1999",
month = "5",
day = "24",
doi = "10.1016/S0304-4165(99)00049-5",
language = "English",
volume = "1427",
pages = "408--416",
journal = "Biochimica et Biophysica Acta - General Subjects",
issn = "0006-3002",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Partial nuclear localization of a bovine phosphoprotein, BCNT, that includes a region derived from a LINE repetitive sequence in Ruminantia

AU - Iwashita, Shintaro

AU - Nobukuni, Takahiro

AU - Tanaka, Shoji

AU - Kobayashi, Mariko

AU - Iwanaga, Toshihiko

AU - Tamate, Hidetoshi B.

AU - Masui, Toru

AU - Takahashi, Ichiro

AU - Hashimoto, Katsuyuki

PY - 1999/5/24

Y1 - 1999/5/24

N2 - BCNT, named after Bucentaur, is a protein that contains a 324-amino-acid region derived from part of a long interspersed DNA sequence element (LINE) in Ruminantia. However, the unique portion is completely missing in human and mouse BCNTs. Since no significant information on their function has been obtained by homology search, we at first examined cellular localization and biochemical characteristics of bovine BCNT to get a hint on its function. Subcellular fractionation and immunohistochemical analyses using a normal bovine epithelial cell line and bovine brain revealed that a significant amount of bovine BCNT is localized in the nuclei, while the major portion is present in the cytosol. Furthermore, it was shown that bovine BCNT is a phosphoprotein and that both bovine and human BCNTs are phosphorylated by casein kinase II in vitro. These results show that BCNTs consist of a unique family, probably a substrate of casein kinase II, which may contribute further to the understanding of gene evolution. Copyright (C) 1999 Elsevier Science B.V.

AB - BCNT, named after Bucentaur, is a protein that contains a 324-amino-acid region derived from part of a long interspersed DNA sequence element (LINE) in Ruminantia. However, the unique portion is completely missing in human and mouse BCNTs. Since no significant information on their function has been obtained by homology search, we at first examined cellular localization and biochemical characteristics of bovine BCNT to get a hint on its function. Subcellular fractionation and immunohistochemical analyses using a normal bovine epithelial cell line and bovine brain revealed that a significant amount of bovine BCNT is localized in the nuclei, while the major portion is present in the cytosol. Furthermore, it was shown that bovine BCNT is a phosphoprotein and that both bovine and human BCNTs are phosphorylated by casein kinase II in vitro. These results show that BCNTs consist of a unique family, probably a substrate of casein kinase II, which may contribute further to the understanding of gene evolution. Copyright (C) 1999 Elsevier Science B.V.

KW - LINE insertion

KW - Nuclear localization

KW - Phosphoprotein

UR - http://www.scopus.com/inward/record.url?scp=0032918061&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032918061&partnerID=8YFLogxK

U2 - 10.1016/S0304-4165(99)00049-5

DO - 10.1016/S0304-4165(99)00049-5

M3 - Article

C2 - 10350657

AN - SCOPUS:0032918061

VL - 1427

SP - 408

EP - 416

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

SN - 0006-3002

IS - 3

ER -