Participation of reactive oxygen species in PGF(2α)-induced apoptosis in rat luteal cells

Mamoru Tanaka, T. Miyazaki, S. Tanigaki, K. Kasai, K. Minegishi, Kei Miyakoshi, H. Ishimoto, Y. Yoshimura

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Prostaglandin F(2α) (PGF(2α)) is implicated in the process of luteal regression in many species. Treatment of rat luteal tissue with PGF(2α) increases the generation of reactive oxygen species. Since reactive oxygen species have been implicated in apoptosis, the present study was undertaken to determine whether reactive oxygen species play a role in the PGF(2α)-induced apoptosis of rat luteal cells. Rat luteal cells were loaded with 6- carboxy-2,7,-dichlorodihydro-fluorescein (CDCFH) diacetate, di (acetomethyl ester), which can be oxidized by reactive oxygen species to yield CDCF, a fluorescent molecule, and the cells were treated with different doses of PGF(2α). Incubation with 100 μmol PGF(2α) I-1 induced an increase in CDCF fluorescence (P<0.05). Treatment of cells with PGF(2α) for 48 h in serum-free medium induced a dose-dependent increase in cell death, and these cells exhibited the morphological characteristics typical of apoptosis, including condensed or fragmented nuclei and fragmentation of internucleosomal DNA. Pretreatment of these cells with ascorbic acid, N,N'-dimethylthiourea, or superoxide dismutase, which acts as an antioxidant or a radical scavenger, prevented the PGF(2α)-induced apoptosis. These results demonstrate that PGF(2α) produces reactive oxygen species and induces apoptosis in rat luteal cells, indicating that the reactive oxygen species may induces apoptosis cell death during luteolysis.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalJournal of Reproduction and Fertility
Volume120
Issue number2
Publication statusPublished - 2000

Fingerprint

Luteal Cells
Prostaglandins F
Reactive Oxygen Species
Apoptosis
Luteolysis
Cell Death
Corpus Luteum
Serum-Free Culture Media
DNA Fragmentation
Ascorbic Acid
Superoxide Dismutase
Esters
Antioxidants
Fluorescence

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Embryology
  • Developmental Biology
  • Molecular Biology
  • Physiology
  • Cell Biology
  • Endocrinology

Cite this

Tanaka, M., Miyazaki, T., Tanigaki, S., Kasai, K., Minegishi, K., Miyakoshi, K., ... Yoshimura, Y. (2000). Participation of reactive oxygen species in PGF(2α)-induced apoptosis in rat luteal cells. Journal of Reproduction and Fertility, 120(2), 239-245.

Participation of reactive oxygen species in PGF(2α)-induced apoptosis in rat luteal cells. / Tanaka, Mamoru; Miyazaki, T.; Tanigaki, S.; Kasai, K.; Minegishi, K.; Miyakoshi, Kei; Ishimoto, H.; Yoshimura, Y.

In: Journal of Reproduction and Fertility, Vol. 120, No. 2, 2000, p. 239-245.

Research output: Contribution to journalArticle

Tanaka, M, Miyazaki, T, Tanigaki, S, Kasai, K, Minegishi, K, Miyakoshi, K, Ishimoto, H & Yoshimura, Y 2000, 'Participation of reactive oxygen species in PGF(2α)-induced apoptosis in rat luteal cells', Journal of Reproduction and Fertility, vol. 120, no. 2, pp. 239-245.
Tanaka, Mamoru ; Miyazaki, T. ; Tanigaki, S. ; Kasai, K. ; Minegishi, K. ; Miyakoshi, Kei ; Ishimoto, H. ; Yoshimura, Y. / Participation of reactive oxygen species in PGF(2α)-induced apoptosis in rat luteal cells. In: Journal of Reproduction and Fertility. 2000 ; Vol. 120, No. 2. pp. 239-245.
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