Parylene-coating in PDMS microfluidic channels prevents the absorption of fluorescent dyes

Hirotaka Sasaki, Hiroaki Onoe, Toshihisa Osaki, Ryuji Kawano, Shoji Takeuchi

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Absorption of fluorescent molecules is a troublesome property of microfluidic devices fabricated with PDMS. The absorption raises background-levels of fluorescence from PDMS, and reduces concentrations of injected fluorescent molecules in PDMS channels. We report here that conformal deposition of poly-p-xylylene derivatives (parylenes) on the surface of PDMS substantially suppressed the absorption of Rhodamine B. In a test of this technique, we found parylene-coated PDMS channels to be useful in accurate analyses using fluorescence intensity.

Original languageEnglish
Pages (from-to)478-482
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume150
Issue number1
DOIs
Publication statusPublished - 2010 Oct 21
Externally publishedYes

Fingerprint

rhodamine B
Fluorescent Dyes
Microfluidics
Dyes
dyes
Fluorescence
coatings
Coatings
Molecules
fluorescence
microfluidic devices
rhodamine
Derivatives
molecules
parylene
poly(p-xylylene)

Keywords

  • Absorption
  • Fluorescence
  • Microfluidic channel
  • Parylene
  • Poly(dimethylsiloxane)

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Parylene-coating in PDMS microfluidic channels prevents the absorption of fluorescent dyes. / Sasaki, Hirotaka; Onoe, Hiroaki; Osaki, Toshihisa; Kawano, Ryuji; Takeuchi, Shoji.

In: Sensors and Actuators, B: Chemical, Vol. 150, No. 1, 21.10.2010, p. 478-482.

Research output: Contribution to journalArticle

Sasaki, Hirotaka ; Onoe, Hiroaki ; Osaki, Toshihisa ; Kawano, Ryuji ; Takeuchi, Shoji. / Parylene-coating in PDMS microfluidic channels prevents the absorption of fluorescent dyes. In: Sensors and Actuators, B: Chemical. 2010 ; Vol. 150, No. 1. pp. 478-482.
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