Patency of heart blood vessels under photosensitization reaction shortly after intravenous injection of talaporfin sodium in canine model

Risa Hamada, Ryota Matsuzaki, Emiyu Ogawa, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In order to investigate patency of heart blood vessels by photosensitization reaction shortly after intravenous injection of talaporfin sodium, we performed in vitro endothelial cell lethality study and in vivo study of heart blood vessel patency in canine one week after photosensitization reaction. Cell lethality of human umbilical vein endothelial cells under different albumin concentrations corresponding with blood and interstice concentrations were employed and their lethality 2 hours after the reaction was measured by WST assay in vitro. Almost all cells survived by 40 J/cm2 photosensitization reaction with blood albumin concentration. Laser diffuser made of plastic optical fiber with 70 mm in length was used in vivo. Red diode laser of 664nm wavelength was emitted from this diffuser with 17.1-42.9 mW/cm in 10 minutes. We estimated the fluence rate distribution by a ray-trace simulator using pre-measured optical coefficients of myocardium tissue, μa; 0.12 mm-1 and μs'; 0.36 mm-1. Almost all blood vessels were patent in every irradiation conditions in canine heart. Coronary artery and vein up to 1 mm diameter were patent in typical myocardium sample with 25.7 mW/cm. We estimated fluence rate distribution of this sample and found that blood vessels were patent even fluence rate over 40 J/cm2. This in vivo study could be explained by the result of in vitro study. We suggest that this blood vessel patency after our particular photosensitization reaction might be because of few photosensitizer uptake in the blood endothelial cells and/or reduced oxidation damage by thick albumin concentration in blood.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XXVII
PublisherSPIE
Volume9706
ISBN (Electronic)9781628419405
DOIs
Publication statusPublished - 2016
EventOptical Interactions with Tissue and Cells XXVII - San Francisco, United States
Duration: 2016 Feb 142016 Feb 17

Other

OtherOptical Interactions with Tissue and Cells XXVII
CountryUnited States
CitySan Francisco
Period16/2/1416/2/17

Keywords

  • blood endothelial cell
  • cell injury
  • patency blood vessel
  • photodynamic therapy
  • photosensitization reaction

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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