Pathogenic epitopes of autoantibodies in pemphigus reside in the amino-terminal adhesive region of desmogleins which are unmasked by proteolytic processing of prosequence

Mariko Yokouchi, Marwah Adly Saleh, Keiko Kuroda, Takahisa Hachiya, John R. Stanley, Masayuki Amagai, Ken Ishii

Research output: Contribution to journalArticle

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Abstract

Pemphigus targets desmogleins (Dsgs), which are thought to be synthesized as inactive precursor proteins with prosequences that are cleaved by substilisin-like proprotein convertases, such as furin, to yield mature adhesive molecules. We hypothesized that some pemphigus pathogenic antibodies (Abs), which presumably interfere with adhesion, only bind the mature form. A pathogenic and three non-pathogenic anti-Dsg1 monoclonal Abs (mAbs) isolated from a pemphigus foliaceus (PF) patient, were used for immunoprecipitation and ELISA of recombinant precursor and mature Dsg1. The pathogenic Ab binds mature Dsg1, whereas non-pathogenic Abs bind either only the precursor or both the precursor and mature Dsg1. Competition ELISA showed that the majority of PF sera target the same or nearby epitopes defined by the pathogenic anti-Dsg1 mAb that blocked 20% binding of 29 out of 40 PF sera. Furthermore, the immunoreactivity of 45 PF sera against the mature Dsg1 was 3.2 fold stronger than that against the precursor Dsg1 by ELISA. Similar results were observed in anti-Dsg3 Abs in 47 pemphigus vulgaris sera, suggesting that most pemphigus sera target epitopes that are unmasked by proteolytic processing. These findings support the idea that at least some pathogenic pemphigus autoantibodies induce the loss of cell adhesion by directly binding the trans-interaction site of Dsgs.

Original languageEnglish
Pages (from-to)2156-2166
Number of pages11
JournalJournal of Investigative Dermatology
Volume129
Issue number9
DOIs
Publication statusPublished - 2009 Sep

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Desmogleins
Pemphigus
Adhesives
Autoantibodies
Epitopes
Antibodies
Processing
Proprotein Convertases
Furin
Protein Precursors
Cell adhesion
Serum
Adhesion
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Molecules
Immunoprecipitation
Cell Adhesion
Anti-Idiotypic Antibodies

ASJC Scopus subject areas

  • Dermatology
  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Pathogenic epitopes of autoantibodies in pemphigus reside in the amino-terminal adhesive region of desmogleins which are unmasked by proteolytic processing of prosequence. / Yokouchi, Mariko; Saleh, Marwah Adly; Kuroda, Keiko; Hachiya, Takahisa; Stanley, John R.; Amagai, Masayuki; Ishii, Ken.

In: Journal of Investigative Dermatology, Vol. 129, No. 9, 09.2009, p. 2156-2166.

Research output: Contribution to journalArticle

Yokouchi, Mariko ; Saleh, Marwah Adly ; Kuroda, Keiko ; Hachiya, Takahisa ; Stanley, John R. ; Amagai, Masayuki ; Ishii, Ken. / Pathogenic epitopes of autoantibodies in pemphigus reside in the amino-terminal adhesive region of desmogleins which are unmasked by proteolytic processing of prosequence. In: Journal of Investigative Dermatology. 2009 ; Vol. 129, No. 9. pp. 2156-2166.
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abstract = "Pemphigus targets desmogleins (Dsgs), which are thought to be synthesized as inactive precursor proteins with prosequences that are cleaved by substilisin-like proprotein convertases, such as furin, to yield mature adhesive molecules. We hypothesized that some pemphigus pathogenic antibodies (Abs), which presumably interfere with adhesion, only bind the mature form. A pathogenic and three non-pathogenic anti-Dsg1 monoclonal Abs (mAbs) isolated from a pemphigus foliaceus (PF) patient, were used for immunoprecipitation and ELISA of recombinant precursor and mature Dsg1. The pathogenic Ab binds mature Dsg1, whereas non-pathogenic Abs bind either only the precursor or both the precursor and mature Dsg1. Competition ELISA showed that the majority of PF sera target the same or nearby epitopes defined by the pathogenic anti-Dsg1 mAb that blocked 20{\%} binding of 29 out of 40 PF sera. Furthermore, the immunoreactivity of 45 PF sera against the mature Dsg1 was 3.2 fold stronger than that against the precursor Dsg1 by ELISA. Similar results were observed in anti-Dsg3 Abs in 47 pemphigus vulgaris sera, suggesting that most pemphigus sera target epitopes that are unmasked by proteolytic processing. These findings support the idea that at least some pathogenic pemphigus autoantibodies induce the loss of cell adhesion by directly binding the trans-interaction site of Dsgs.",
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