Pathogenic monoclonal antibody against desmoglein 3 augments desmoglein 3 and p38 MAPK phosphorylation in human squamous carcinoma cell line

Yuki Kawasaki, Yumi Aoyama, Kazuyuki Tsunoda, Masayuki Amagai, Yasuo Kitajima

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Pemphigus vulgaris is an autoimmune blistering disease characterized by cell-cell detachment of epidermal cells. Autoantibody against desmoglein (Dsg) 3, a transmembrane glycoprotein that mediates the association of desmosomes, plays a major role in blistering in pemphigus vulgaris (PV). The mechanisms of autoantibody-induced acantholysis have not been clarified. We previously reported that PV-IgG induces phosphorylation of Dsg3, decreases Dsg3 on the cell surface and forms Dsg3-depleted desmosomes in cultured keratinocytes, and that cell treatment with a potent pathogenic monoclonal antibody against Dsg3 (AK23 mAb) decreases the amount of Dsg3 in cultured keratinocytes. Although the precise mechanisms remain unclear, we have proposed the involvement of intracellular signal transduction resulting from the binding of autoantibodies to Dsg3. In this study, we examined whether AK23 mAb augments phosphorylation of Dsg3 and p38 mitogen-activating protein kinase (MAPK) in a human squamous cell line, DJM-1 cells. AK23 mAb increased serine phosphorylation of Dsg3 and augmented activation levels of p38 MAPK. These results indicate that antibodies bind to Dsg3, but not other antigens, in the IgG fraction and can induce activation of signal transduction.

Original languageEnglish
Pages (from-to)587-590
Number of pages4
JournalAutoimmunity
Volume39
Issue number7
DOIs
Publication statusPublished - 2006 Nov

Fingerprint

Desmoglein 3
Mitogens
Protein Kinases
Squamous Cell Carcinoma
Monoclonal Antibodies
Phosphorylation
Pemphigus
Cell Line
Autoantibodies
Desmosomes
Keratinocytes
Signal Transduction
Immunoglobulin G
Acantholysis
Serine
Autoimmune Diseases
Cultured Cells
Glycoproteins
Epithelial Cells
Antigens

Keywords

  • Desmoglein
  • Desmosome
  • Keratinocyte
  • Pemphigus
  • Phosphorylation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Pathogenic monoclonal antibody against desmoglein 3 augments desmoglein 3 and p38 MAPK phosphorylation in human squamous carcinoma cell line. / Kawasaki, Yuki; Aoyama, Yumi; Tsunoda, Kazuyuki; Amagai, Masayuki; Kitajima, Yasuo.

In: Autoimmunity, Vol. 39, No. 7, 11.2006, p. 587-590.

Research output: Contribution to journalArticle

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