Pattern of fin rays along the antero-posterior axis based on their connection to distal radials

Hiroki Hamada, Toshiaki Uemoto, Yoshitaka Tanaka, Yuki Honda, Keiichi Kitajima, Tetsuya Umeda, Atsushi Kawakami, Minori Shinya, Koichi Kawakami, Koji Tamura, Gembu Abe

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Teleost paired fins are composed of two endoskeletal domains, proximal and distal radials, and an exoskeletal domain, the fin ray. The zebrafish pectoral fin displays elaborately patterned radials along the anteroposterior (AP) axis. Radials are considered homologous to tetrapod limb skeletons, and their patterning mechanisms in embryonic development are similar to those of limb development. Nevertheless, the pattern along the AP axis in fin rays has not been well described in the zebrafish pectoral fin, although several recent reports have revealed that fin ray development shares some cellular and genetic properties with fin/limb endoskeleton development. Thus, fin ray morphogenesis may involve developmental mechanisms for AP patterning in the fin/limb endoskeleton, and may have a specific pattern along the AP axis. Results: We conducted detailed morphological observations on fin rays and their connection to distal radials by comparing intra-and inter-strain zebrafish specimens. Although the number of fin rays varied, pectoral fin rays could be categorized into three domains along the AP axis, according to the connection between the fin rays and distal radials; additionally, the number of fin rays varied in the posterior part of the three domains. This result was confirmed by observation of the morphogenesis process of fin rays and distal radials, which showed altered localization of distal radials in the middle domain. We also evaluated the expression pattern of lhx genes, which have AP patterning activity in limb development, in fin rays and during distal radial development and found these genes to be expressed during morphogenesis in both fin rays and distal radials. Conclusion: The fin ray and its connection to the endoskeleton are patterned along the AP axis, and the pattern along the AP axis in the fin ray and the radial connection is constructed by the developmental mechanism related to AP patterning in the limb/fin bud. Our results indicate the possibility that the developmental mechanisms of fin rays and their connection are comparable to those of the distal element of the limb skeleton.

Original languageEnglish
Article number30
JournalZoological Letters
Volume5
Issue number1
DOIs
Publication statusPublished - 2019 Sep 18

Keywords

  • Antero-posterior patterning
  • Fin ray
  • IM-II
  • Phenotypic variation
  • Zebrafish strains

ASJC Scopus subject areas

  • Animal Science and Zoology

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  • Cite this

    Hamada, H., Uemoto, T., Tanaka, Y., Honda, Y., Kitajima, K., Umeda, T., Kawakami, A., Shinya, M., Kawakami, K., Tamura, K., & Abe, G. (2019). Pattern of fin rays along the antero-posterior axis based on their connection to distal radials. Zoological Letters, 5(1), [30]. https://doi.org/10.1186/s40851-019-0145-z