Pax-6 is required for thalamocortical pathway formation in fetal rats

Hitoshi Kawano, Tetsuya Fukuda, Kenichiro Kubo, Masao Horie, Keiichi Uyemura, Kosei Takeuchi, Noriko Osumi, Kazuhiro Eto, Koki Kawamura

Research output: Contribution to journalArticle

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Abstract

Pax-6, a transcription regulatory factor, has been demonstrated to play important roles in eye, nose, and brain development by analyzing mice, rats, and humans with a Pax-6 gene mutation. We examined the role of Pax-6 with special attention to the formation of efferent and afferent pathways of the cerebral cortex by using the rat Small eye (rSey2), which has a mutation in the Pax-6 gene. In rSey2/rSey2 fetuses, cortical efferent axons develop with normal trajectory, at least within the cortical anlage, when examined with immunohistochemistry of the neuronal cell adhesion molecule TAG-1 and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) labeling from the cortical surface. A remarkable disorder was found in the trajectory of dorsal thalamic axons by immunostaining of the neurofilament and the neural cell adhesion molecule L1 and DiI labeling from the dorsal thalamus. In normal rat fetuses, dorsal thalamic axons curved laterally in the ventral thalamus without invading a Pax-6-immunoreactive cell cluster in the ventral part of the ventral thalamus. These axons then coursed up to the cortical anlage, passing just dorsal to another Pax-6-immunoreactive cell cluster in the amygdaloid region. In contrast, in rSey2/rSey2 fetuses, dorsal thalamic axons extended downward to converge in the ventrolateral corner of the ventral thalamus and fanned out in the amygdaloid region without reaching the cortical anlage. These results suggest that Pax-6- expressing cell clusters along the thalamocortical pathway (ventral part of the ventral thalamus and amygdala) are responsible for the determination of the axonal pathfinding of the thalamocortical pathway.

Original languageEnglish
Pages (from-to)147-160
Number of pages14
JournalJournal of Comparative Neurology
Volume408
Issue number2
DOIs
Publication statusPublished - 1999 May 31

Fingerprint

Thalamus
Axons
Fetus
Neuronal Cell Adhesion Molecules
Neural Cell Adhesion Molecule L1
Afferent Pathways
Efferent Pathways
Mutation
Intermediate Filaments
Amygdala
Nose
Cerebral Cortex
Genes
Transcription Factors
Immunohistochemistry
Brain

Keywords

  • Amygdala
  • DiI
  • Immunohistochemistry
  • L1
  • Mutant
  • Thalamus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Pax-6 is required for thalamocortical pathway formation in fetal rats. / Kawano, Hitoshi; Fukuda, Tetsuya; Kubo, Kenichiro; Horie, Masao; Uyemura, Keiichi; Takeuchi, Kosei; Osumi, Noriko; Eto, Kazuhiro; Kawamura, Koki.

In: Journal of Comparative Neurology, Vol. 408, No. 2, 31.05.1999, p. 147-160.

Research output: Contribution to journalArticle

Kawano, H, Fukuda, T, Kubo, K, Horie, M, Uyemura, K, Takeuchi, K, Osumi, N, Eto, K & Kawamura, K 1999, 'Pax-6 is required for thalamocortical pathway formation in fetal rats', Journal of Comparative Neurology, vol. 408, no. 2, pp. 147-160. https://doi.org/10.1002/(SICI)1096-9861(19990531)408:2<147::AID-CNE1>3.0.CO;2-L
Kawano, Hitoshi ; Fukuda, Tetsuya ; Kubo, Kenichiro ; Horie, Masao ; Uyemura, Keiichi ; Takeuchi, Kosei ; Osumi, Noriko ; Eto, Kazuhiro ; Kawamura, Koki. / Pax-6 is required for thalamocortical pathway formation in fetal rats. In: Journal of Comparative Neurology. 1999 ; Vol. 408, No. 2. pp. 147-160.
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