pc1 and psc1, zebrafish homologs of Drosophila Polycomb and Posterior sex combs, encode nuclear proteins capable of complex interactions

Akinori Kawamura, Shin'ichiro Yokota, Kouji Yamada, Hitoshi Inoue, Keiji Inohaya, Ken Yamazaki, Ikuo Yasumasu, Toru Higashinakagawa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Drosophila Polycomb group proteins are thought to form multimeric nuclear complexes that are responsible for stable transmission of repressed states of gene expression during the proliferation of differentiating embryos. In this study, we cloned, sequenced, and characterized two Polycomb group homologs, designated pc1 and psc1, in zebrafish. Amino acid sequence analyses determined that pc1 is a structural homolog of Drosophila Polycomb and that psc1 is a homolog of Drosophila Posterior sex combs. Northern blots and whole-mount in situ hybridization revealed that pc1 and psc1 had overlapping expression patterns at successive stages of embryogenesis. Immunocytochemistry localized both Pc1 and Psc1 protein in blastomere nuclei. Pull-down assays and two-hybrid system deletion analyses showed that these proteins were capable of homotypic and heterotypic interactions and identified the regions required for these interactions. The evidence supports the idea that zebrafish Polycomb group proteins, like those of other species, form nuclear complexes with compositions that may vary in a spatio-temporal manner during development.

Original languageEnglish
Pages (from-to)456-463
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume294
Issue number2
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Polycomb-Group Proteins
Comb and Wattles
Zebrafish
Nuclear Proteins
Drosophila
Zebrafish Proteins
Blastomeres
Two-Hybrid System Techniques
Protein Sequence Analysis
Hybrid systems
Gene expression
Northern Blotting
Embryonic Development
In Situ Hybridization
Assays
Proteins
Embryonic Structures
Immunohistochemistry
Gene Expression
Amino Acids

Keywords

  • Gene expression
  • Nuclear complex
  • Polycomb group
  • Protein-protein interactions
  • Zebrafish

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

pc1 and psc1, zebrafish homologs of Drosophila Polycomb and Posterior sex combs, encode nuclear proteins capable of complex interactions. / Kawamura, Akinori; Yokota, Shin'ichiro; Yamada, Kouji; Inoue, Hitoshi; Inohaya, Keiji; Yamazaki, Ken; Yasumasu, Ikuo; Higashinakagawa, Toru.

In: Biochemical and Biophysical Research Communications, Vol. 294, No. 2, 2002, p. 456-463.

Research output: Contribution to journalArticle

Kawamura, Akinori ; Yokota, Shin'ichiro ; Yamada, Kouji ; Inoue, Hitoshi ; Inohaya, Keiji ; Yamazaki, Ken ; Yasumasu, Ikuo ; Higashinakagawa, Toru. / pc1 and psc1, zebrafish homologs of Drosophila Polycomb and Posterior sex combs, encode nuclear proteins capable of complex interactions. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 294, No. 2. pp. 456-463.
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AU - Inoue, Hitoshi

AU - Inohaya, Keiji

AU - Yamazaki, Ken

AU - Yasumasu, Ikuo

AU - Higashinakagawa, Toru

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