PDGFBB promotes PDGFRα-positive cell migration into artificial bone in vivo

Shigeyuki Yoshida; Ryotaro Iwasaki; Hiromasa Kawana; Yoshiteru Miyauchi; Hiroko Hoshi; Hiroya Miyamoto; Tomoaki Mori; Hiroya Kanagawa; Eri Katsuyama; Atsuhiro Fujie; Wu Hao; Tami Kobayashi; Yuiko Sato; Kana Miyamoto; Hideo Morioka; Morio Matsumoto; Kazuhiro Chiba; Yoshiaki Toyama; Taneaki Nakagawa; Takeshi Miyamoto

doi:10.1016/j.bbrc.2012.04.084

PDGFBB promotes PDGFRα-positive cell migration into artificial bone in vivo

Shigeyuki Yoshida, Ryotaro Iwasaki, Hiromasa Kawana, Yoshiteru Miyauchi, Hiroko Hoshi, Hiroya Miyamoto, Tomoaki Mori, Hiroya Kanagawa, Eri Katsuyama, Atsuhiro Fujie, Wu Hao, Tami Kobayashi, Yuiko Sato, Kana Miyamoto, Hideo Morioka, Morio Matsumoto, Kazuhiro Chiba, Yoshiaki Toyama, Taneaki Nakagawa, Takeshi Miyamoto

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Bone defects caused by traumatic bone loss or tumor dissection are now treated with auto- or allo-bone graft, and also occasionally by artificial bone transplantation, particularly in the case of large bone defects. However, artificial bones often exhibit poor affinity to host bones followed by bony union failure. Thus therapies combining artificial bones with growth factors have been sought. Here we report that platelet derived growth factor bb (PDGFBB) promotes a significant increase in migration of PDGF receptor α (PDGFRα)-positive mesenchymal stem cells/pre-osteoblastic cells into artificial bone . in vivo. Growth factors such as transforming growth factor beta (TGFβ) and hepatocyte growth factor (HGF) reportedly inhibit osteoblast differentiation; however, PDGFBB did not exhibit such inhibitory effects and in fact stimulated osteoblast differentiation . in vitro, suggesting that combining artificial bones with PDGFBB treatment could promote host cell migration into artificial bones without inhibiting osteoblastogenesis.

Original language	English
Pages (from-to)	785-789
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	421
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2012.04.084
Publication status	Published - 2012 May 18

Keywords

Artificial bone
Bone
Migration
Osteoblasts
PDGFBB

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2012.04.084

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Yoshida, S., Iwasaki, R., Kawana, H., Miyauchi, Y., Hoshi, H., Miyamoto, H., Mori, T., Kanagawa, H., Katsuyama, E., Fujie, A., Hao, W., Kobayashi, T., Sato, Y., Miyamoto, K., Morioka, H., Matsumoto, M., Chiba, K., Toyama, Y., Nakagawa, T., & Miyamoto, T. (2012). PDGFBB promotes PDGFRα-positive cell migration into artificial bone in vivo. Biochemical and Biophysical Research Communications, 421(4), 785-789. https://doi.org/10.1016/j.bbrc.2012.04.084

Yoshida, S, Iwasaki, R, Kawana, H, Miyauchi, Y, Hoshi, H, Miyamoto, H, Mori, T, Kanagawa, H, Katsuyama, E, Fujie, A, Hao, W, Kobayashi, T, Sato, Y, Miyamoto, K, Morioka, H, Matsumoto, M, Chiba, K, Toyama, Y, Nakagawa, T & Miyamoto, T 2012, 'PDGFBB promotes PDGFRα-positive cell migration into artificial bone in vivo', Biochemical and Biophysical Research Communications, vol. 421, no. 4, pp. 785-789. https://doi.org/10.1016/j.bbrc.2012.04.084

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title = "PDGFBB promotes PDGFRα-positive cell migration into artificial bone in vivo",

abstract = "Bone defects caused by traumatic bone loss or tumor dissection are now treated with auto- or allo-bone graft, and also occasionally by artificial bone transplantation, particularly in the case of large bone defects. However, artificial bones often exhibit poor affinity to host bones followed by bony union failure. Thus therapies combining artificial bones with growth factors have been sought. Here we report that platelet derived growth factor bb (PDGFBB) promotes a significant increase in migration of PDGF receptor α (PDGFRα)-positive mesenchymal stem cells/pre-osteoblastic cells into artificial bone . in vivo. Growth factors such as transforming growth factor beta (TGFβ) and hepatocyte growth factor (HGF) reportedly inhibit osteoblast differentiation; however, PDGFBB did not exhibit such inhibitory effects and in fact stimulated osteoblast differentiation . in vitro, suggesting that combining artificial bones with PDGFBB treatment could promote host cell migration into artificial bones without inhibiting osteoblastogenesis.",

keywords = "Artificial bone, Bone, Migration, Osteoblasts, PDGFBB",

author = "Shigeyuki Yoshida and Ryotaro Iwasaki and Hiromasa Kawana and Yoshiteru Miyauchi and Hiroko Hoshi and Hiroya Miyamoto and Tomoaki Mori and Hiroya Kanagawa and Eri Katsuyama and Atsuhiro Fujie and Wu Hao and Tami Kobayashi and Yuiko Sato and Kana Miyamoto and Hideo Morioka and Morio Matsumoto and Kazuhiro Chiba and Yoshiaki Toyama and Taneaki Nakagawa and Takeshi Miyamoto",

note = "Funding Information: This work was partially supported by a Grant for Standardization of non-operative treatment and minimum invasion surgery for osteoporotic vertebral fracture. We thank Dr. H. Inoue for providing βTCP (Olympus Terumo Biomaterials Corporation). S. Yoshida was supported by the Global Center of Excellence Program and Ishidsu Shun Memorial Scholarship. T. Miyamoto was supported by a Grant-in-aid for Young Scientists (A) and the Global Center of Excellence Program, the Takeda Science Foundation, the Nakatomi Foundation, the Inamori Foundation and the Keio Kanrinmaru project, Japan. The authors have no conflicting financial interests.",

year = "2012",

month = may,

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doi = "10.1016/j.bbrc.2012.04.084",

language = "English",

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T1 - PDGFBB promotes PDGFRα-positive cell migration into artificial bone in vivo

AU - Yoshida, Shigeyuki

AU - Iwasaki, Ryotaro

AU - Kawana, Hiromasa

AU - Miyauchi, Yoshiteru

AU - Hoshi, Hiroko

AU - Miyamoto, Hiroya

AU - Mori, Tomoaki

AU - Kanagawa, Hiroya

AU - Katsuyama, Eri

AU - Fujie, Atsuhiro

AU - Hao, Wu

AU - Kobayashi, Tami

AU - Sato, Yuiko

AU - Miyamoto, Kana

AU - Morioka, Hideo

AU - Matsumoto, Morio

AU - Chiba, Kazuhiro

AU - Toyama, Yoshiaki

AU - Nakagawa, Taneaki

AU - Miyamoto, Takeshi

N1 - Funding Information: This work was partially supported by a Grant for Standardization of non-operative treatment and minimum invasion surgery for osteoporotic vertebral fracture. We thank Dr. H. Inoue for providing βTCP (Olympus Terumo Biomaterials Corporation). S. Yoshida was supported by the Global Center of Excellence Program and Ishidsu Shun Memorial Scholarship. T. Miyamoto was supported by a Grant-in-aid for Young Scientists (A) and the Global Center of Excellence Program, the Takeda Science Foundation, the Nakatomi Foundation, the Inamori Foundation and the Keio Kanrinmaru project, Japan. The authors have no conflicting financial interests.

PY - 2012/5/18

Y1 - 2012/5/18

N2 - Bone defects caused by traumatic bone loss or tumor dissection are now treated with auto- or allo-bone graft, and also occasionally by artificial bone transplantation, particularly in the case of large bone defects. However, artificial bones often exhibit poor affinity to host bones followed by bony union failure. Thus therapies combining artificial bones with growth factors have been sought. Here we report that platelet derived growth factor bb (PDGFBB) promotes a significant increase in migration of PDGF receptor α (PDGFRα)-positive mesenchymal stem cells/pre-osteoblastic cells into artificial bone . in vivo. Growth factors such as transforming growth factor beta (TGFβ) and hepatocyte growth factor (HGF) reportedly inhibit osteoblast differentiation; however, PDGFBB did not exhibit such inhibitory effects and in fact stimulated osteoblast differentiation . in vitro, suggesting that combining artificial bones with PDGFBB treatment could promote host cell migration into artificial bones without inhibiting osteoblastogenesis.

AB - Bone defects caused by traumatic bone loss or tumor dissection are now treated with auto- or allo-bone graft, and also occasionally by artificial bone transplantation, particularly in the case of large bone defects. However, artificial bones often exhibit poor affinity to host bones followed by bony union failure. Thus therapies combining artificial bones with growth factors have been sought. Here we report that platelet derived growth factor bb (PDGFBB) promotes a significant increase in migration of PDGF receptor α (PDGFRα)-positive mesenchymal stem cells/pre-osteoblastic cells into artificial bone . in vivo. Growth factors such as transforming growth factor beta (TGFβ) and hepatocyte growth factor (HGF) reportedly inhibit osteoblast differentiation; however, PDGFBB did not exhibit such inhibitory effects and in fact stimulated osteoblast differentiation . in vitro, suggesting that combining artificial bones with PDGFBB treatment could promote host cell migration into artificial bones without inhibiting osteoblastogenesis.

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KW - Migration

KW - Osteoblasts

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ER -

PDGFBB promotes PDGFRα-positive cell migration into artificial bone in vivo

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Keywords

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