PDGFRα-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1) to regenerate injured epithelia

Katsuto Tamai; Takehiko Yamazaki; Takenao Chino; Masaru Ishii; Satoru Otsuru; Yasushi Kikuchi; Shin Iinuma; Kotaro Saga; Keisuke Nimura; Takashi Shimbo; Noriko Umegaki; Ichiro Katayama; Jun Ichi Miyazaki; Junji Takeda; John A. McGrath; Jouni Uitto; Yasufumi Kaneda

doi:10.1073/pnas.1016753108

PDGFRα-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1) to regenerate injured epithelia

Katsuto Tamai, Takehiko Yamazaki, Takenao Chino, Masaru Ishii, Satoru Otsuru, Yasushi Kikuchi, Shin Iinuma, Kotaro Saga, Keisuke Nimura, Takashi Shimbo, Noriko Umegaki, Ichiro Katayama, Jun Ichi Miyazaki, Junji Takeda, John A. McGrath, Jouni Uitto, Yasufumi Kaneda

Research output: Contribution to journal › Article › peer-review

183 Citations (Scopus)

Abstract

The role of bone marrow cells in repairing ectodermal tissue, such as skin epidermis, is not clear. To explore this process further, this study examined a particular form of cutaneous repair, skin grafting. Grafting of full thickness wild-type mouse skin onto mice that had received a green fluorescent protein-bone marrow transplant after whole body irradiation led to an abundance of bone marrow-derived epithelial cells in follicular and interfollicular epidermis that persisted for at least 5 mo. The source of the epithelial progenitors was the nonhematopoietic, platelet-derived growth factor receptor α-positive (Lin^-/PDGFRα⁺) bone marrow cell population. Skin grafts release high mobility group box 1 (HMGB1) in vitro and in vivo, which can mobilize the Lin^-/PDGFRα⁺ cells from bone marrow to target the engrafted skin. These data provide unique insight into how skin grafts facilitate tissue repair and identify strategies germane to regenerative medicine for skin and, perhaps, other ectodermal defects or diseases.

Original language	English
Pages (from-to)	6609-6614
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	108
Issue number	16
DOIs	https://doi.org/10.1073/pnas.1016753108
Publication status	Published - 2011 Apr 19
Externally published	Yes

Keywords

Epidermolysis bullosa
Keratinocyte
Skin injury
Stem cells
Tissue regeneration

ASJC Scopus subject areas

General

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10.1073/pnas.1016753108

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Tamai, K., Yamazaki, T., Chino, T., Ishii, M., Otsuru, S., Kikuchi, Y., Iinuma, S., Saga, K., Nimura, K., Shimbo, T., Umegaki, N., Katayama, I., Miyazaki, J. I., Takeda, J., McGrath, J. A., Uitto, J., & Kaneda, Y. (2011). PDGFRα-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1) to regenerate injured epithelia. Proceedings of the National Academy of Sciences of the United States of America, 108(16), 6609-6614. https://doi.org/10.1073/pnas.1016753108

PDGFRα-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1) to regenerate injured epithelia. / Tamai, Katsuto; Yamazaki, Takehiko; Chino, Takenao et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 16, 19.04.2011, p. 6609-6614.

Research output: Contribution to journal › Article › peer-review

Tamai, K, Yamazaki, T, Chino, T, Ishii, M, Otsuru, S, Kikuchi, Y, Iinuma, S, Saga, K, Nimura, K, Shimbo, T, Umegaki, N, Katayama, I, Miyazaki, JI, Takeda, J, McGrath, JA, Uitto, J & Kaneda, Y 2011, 'PDGFRα-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1) to regenerate injured epithelia', Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 16, pp. 6609-6614. https://doi.org/10.1073/pnas.1016753108

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title = "PDGFRα-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1) to regenerate injured epithelia",

abstract = "The role of bone marrow cells in repairing ectodermal tissue, such as skin epidermis, is not clear. To explore this process further, this study examined a particular form of cutaneous repair, skin grafting. Grafting of full thickness wild-type mouse skin onto mice that had received a green fluorescent protein-bone marrow transplant after whole body irradiation led to an abundance of bone marrow-derived epithelial cells in follicular and interfollicular epidermis that persisted for at least 5 mo. The source of the epithelial progenitors was the nonhematopoietic, platelet-derived growth factor receptor α-positive (Lin-/PDGFRα+) bone marrow cell population. Skin grafts release high mobility group box 1 (HMGB1) in vitro and in vivo, which can mobilize the Lin-/PDGFRα+ cells from bone marrow to target the engrafted skin. These data provide unique insight into how skin grafts facilitate tissue repair and identify strategies germane to regenerative medicine for skin and, perhaps, other ectodermal defects or diseases.",

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AU - Takeda, Junji

AU - McGrath, John A.

AU - Uitto, Jouni

AU - Kaneda, Yasufumi

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AB - The role of bone marrow cells in repairing ectodermal tissue, such as skin epidermis, is not clear. To explore this process further, this study examined a particular form of cutaneous repair, skin grafting. Grafting of full thickness wild-type mouse skin onto mice that had received a green fluorescent protein-bone marrow transplant after whole body irradiation led to an abundance of bone marrow-derived epithelial cells in follicular and interfollicular epidermis that persisted for at least 5 mo. The source of the epithelial progenitors was the nonhematopoietic, platelet-derived growth factor receptor α-positive (Lin-/PDGFRα+) bone marrow cell population. Skin grafts release high mobility group box 1 (HMGB1) in vitro and in vivo, which can mobilize the Lin-/PDGFRα+ cells from bone marrow to target the engrafted skin. These data provide unique insight into how skin grafts facilitate tissue repair and identify strategies germane to regenerative medicine for skin and, perhaps, other ectodermal defects or diseases.

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PDGFRα-positive cells in bone marrow are mobilized by high mobility group box 1 (HMGB1) to regenerate injured epithelia

Abstract

Keywords

ASJC Scopus subject areas

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