Pemphigus vulgaris antigen (Desmoglein 3) is localized in the lower epidermis, the site of blister formation in patients

Masayuki Amagai, Peter J. Koch, Takeji Nishikawa, John R. Stanley

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

In patients with pemphigus vulgaris, autoantibodies against the desmosomal glycoprotein desmoglein 3 (Dsg3) cause blisters due to loss of keratinocyte cell-cell adhesion in the basal and immediate suprabasal layer of the deeper epidermis, leaving the superficial epidermis intact. Autoantibodies from these patients, however, usually bind to the cell surface of keratinocytes throughout the entire epidermis, as determined by indirect immunofluorescence. To explain this apparent paradox, we immunoadsorbed pemphigus vulgaris sera with the extracellular domains of Dsg3 and desmoglein 1 (Dsg1) produced by insect cells infected with recombinant baculovirus. When adsorbed with extracellular domains of both Dsg3 and Dsg1, these sera no longer stained epidermis, demonstrating that most, if not all, of their cell surface reactivity can be attributed to antibodies against the extracellular domains of these desmogleins. Adsorption with only the Dsg1 extracellular domain left antibodies that stained only the basal and immediate suprabasal layers of the epidermis and immunoprecipitated only Dsg3, not Dsg1, from extracts of cultured cells synthesizing these molecules, In contrast, adsorption with only the Dsg3 extracellular domain left antibodies that stained only the more superficial epidermis and immunoprecipitated only Dsg1, These data localize Dsg3 exactly to the area in the epidermis where blisters occur in pemphigus vulgaris.

Original languageEnglish
Pages (from-to)351-355
Number of pages5
JournalJournal of Investigative Dermatology
Volume106
Issue number2
Publication statusPublished - 1996

Fingerprint

Desmoglein 3
Desmoglein 1
Pemphigus
Blister
Epidermis
Antigens
Autoantibodies
Antibodies
Keratinocytes
Desmogleins
Adsorption
Cell adhesion
Baculoviridae
Indirect Fluorescent Antibody Technique
Serum
Glycoproteins
Cell Adhesion
Insects
Cells
Cultured Cells

Keywords

  • Autoantibodies
  • Cadherins
  • Cell adhesion
  • Desmosomes

ASJC Scopus subject areas

  • Dermatology

Cite this

Pemphigus vulgaris antigen (Desmoglein 3) is localized in the lower epidermis, the site of blister formation in patients. / Amagai, Masayuki; Koch, Peter J.; Nishikawa, Takeji; Stanley, John R.

In: Journal of Investigative Dermatology, Vol. 106, No. 2, 1996, p. 351-355.

Research output: Contribution to journalArticle

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