Abstract
Because pemphigus vulgaris antigen (PVA) is homologous to desmogleins which are in desmosomes, we asked whether PVA is also localized to desmosomes. Immunogold electron microscopy was used to localize binding of PV antibodies. Rabbit antibodies raised against fusion proteins containing the extracellular portion of PVA bound to desmosomes of cultured keratinocytes. PV patient serum affinity-purified on one of these fusion proteins and injected into neonatal mice localized to separating desmosomes. These results show that PVA belongs to the desmoglein subfamily of cadherins according to sequence, localization and, presumably, function.
| Original language | English |
|---|---|
| Pages (from-to) | 24-26 |
| Number of pages | 3 |
| Journal | Dermatology |
| Volume | 189 |
| Issue number | SUPPL. 1 |
| Publication status | Published - 1994 |
| Externally published | Yes |
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Keywords
- Cadherins
- Desmoglein
- Desmosomes
- Pemphigus vulgaris antigen
ASJC Scopus subject areas
- Dermatology
Cite this
Pemphigus vulgaris antigen is a desmosomal desmoglein. / Karpati, S.; Amagai, Masayuki; Prussick, R.; Stanley, J. R.
In: Dermatology, Vol. 189, No. SUPPL. 1, 1994, p. 24-26.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pemphigus vulgaris antigen is a desmosomal desmoglein
AU - Karpati, S.
AU - Amagai, Masayuki
AU - Prussick, R.
AU - Stanley, J. R.
PY - 1994
Y1 - 1994
N2 - Because pemphigus vulgaris antigen (PVA) is homologous to desmogleins which are in desmosomes, we asked whether PVA is also localized to desmosomes. Immunogold electron microscopy was used to localize binding of PV antibodies. Rabbit antibodies raised against fusion proteins containing the extracellular portion of PVA bound to desmosomes of cultured keratinocytes. PV patient serum affinity-purified on one of these fusion proteins and injected into neonatal mice localized to separating desmosomes. These results show that PVA belongs to the desmoglein subfamily of cadherins according to sequence, localization and, presumably, function.
AB - Because pemphigus vulgaris antigen (PVA) is homologous to desmogleins which are in desmosomes, we asked whether PVA is also localized to desmosomes. Immunogold electron microscopy was used to localize binding of PV antibodies. Rabbit antibodies raised against fusion proteins containing the extracellular portion of PVA bound to desmosomes of cultured keratinocytes. PV patient serum affinity-purified on one of these fusion proteins and injected into neonatal mice localized to separating desmosomes. These results show that PVA belongs to the desmoglein subfamily of cadherins according to sequence, localization and, presumably, function.
KW - Cadherins
KW - Desmoglein
KW - Desmosomes
KW - Pemphigus vulgaris antigen
UR - http://www.scopus.com/inward/record.url?scp=0028346770&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028346770&partnerID=8YFLogxK
M3 - Article
C2 - 8049558
AN - SCOPUS:0028346770
VL - 189
SP - 24
EP - 26
JO - Dermatology
JF - Dermatology
SN - 1018-8665
IS - SUPPL. 1
ER -