Peptides bound to stereoregular PMMAs

Toshiki Sawada, Hisao Matsuno, Teruhiko Matsubara, Toshinori Sato, Tatsuki Kitayama, Takeshi Serizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To identify peptides with selective affinity for stereoregular isotactic poly(methyl methacrylate) (it-PMMA), the phage display selection using a heptamer random peptide library was carried out. After five rounds of biopanning, several phage clones with high specific affinity to it-PMMA were selected. As a result of DNA sequencing, nine kinds of it-PMMA binding phage clones were isolated. The binding affinity and specificity of these phage clones were quantitatively investigated by an enzyme-linked immunosorbent assay. These clones not only bound to it-PMMA greater than a phage library, but showed the specific binding to it-PMMA rather than syndiotactic and atactic PMMAs.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan
Pages4867-4868
Number of pages2
Volume54
Edition2
Publication statusPublished - 2005
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

Fingerprint

Bacteriophages
Polymethyl methacrylates
Peptides
Assays
DNA
Enzymes
Display devices

Keywords

  • Peptide
  • Phage display
  • Poly(methyl methacrylate)
  • Tacticity

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Sawada, T., Matsuno, H., Matsubara, T., Sato, T., Kitayama, T., & Serizawa, T. (2005). Peptides bound to stereoregular PMMAs. In Polymer Preprints, Japan (2 ed., Vol. 54, pp. 4867-4868)

Peptides bound to stereoregular PMMAs. / Sawada, Toshiki; Matsuno, Hisao; Matsubara, Teruhiko; Sato, Toshinori; Kitayama, Tatsuki; Serizawa, Takeshi.

Polymer Preprints, Japan. Vol. 54 2. ed. 2005. p. 4867-4868.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sawada, T, Matsuno, H, Matsubara, T, Sato, T, Kitayama, T & Serizawa, T 2005, Peptides bound to stereoregular PMMAs. in Polymer Preprints, Japan. 2 edn, vol. 54, pp. 4867-4868, 54th SPSJ Symposium on Macromolecules, Yamagata, Japan, 05/9/20.
Sawada T, Matsuno H, Matsubara T, Sato T, Kitayama T, Serizawa T. Peptides bound to stereoregular PMMAs. In Polymer Preprints, Japan. 2 ed. Vol. 54. 2005. p. 4867-4868
Sawada, Toshiki ; Matsuno, Hisao ; Matsubara, Teruhiko ; Sato, Toshinori ; Kitayama, Tatsuki ; Serizawa, Takeshi. / Peptides bound to stereoregular PMMAs. Polymer Preprints, Japan. Vol. 54 2. ed. 2005. pp. 4867-4868
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