Peptidomic analysis of the central nervous system of the protochordate, Ciona intestinalis: Homologs and prototypes of vertebrate peptides and novel peptides

Tsuyoshi Kawada, Michio Ogasawara, Toshio Sekiguchi, Masato Aoyama, Kohji Hotta, Kotaro Oka, Honoo Satake

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The phylogenetic position of ascidians as the chordate invertebrates closest to vertebrates suggests that they might possess homologs and/or prototypes of vertebrate peptide hormones and neuropeptides as well as ascidian-specific peptides. However, only a small number of peptides have so far been identified in ascidians. In the present study, we have identified various peptides in the ascidian, Ciona intestinalis. Mass spectrometry-based peptidomic analysis detected 33 peptides, including 26 novel peptides, from C. intestinalis. The ascidian peptides are largely classified into three categories: 1) prototypes and homologs of vertebrate peptides, such as galanin/galanin-like peptide, which have never been identified in any invertebrates; 2) peptides partially homologous with vertebrate peptides, including novel neurotesin-like peptides; 3) novel peptides. These results not only provide evidence that C. intestinalis possesses various homologs and prototypes of vertebrate neuropeptides and peptide hormones but also suggest that several of these peptides might have diverged in the ascidian-specific evolutionary lineage. All Ciona peptide genes were expressed in the neural complex, where as several peptide gene transcripts were also distributed in peripheral tissues, including the ovary. Furthermore, a Ciona neurotensin-like peptide, C. intestinalis neurotensin-like peptide 6, was shown to down-regulate growth of Ciona vitellogenic oocytes. These results suggest that the Ciona peptides act not only as neuropeptides in the neural tissue but also as hormones in nonneuronal tissues and that ascidians, unlike other invertebrates, such as nematodes, insects, and sea urchins, established an evolutionary origin of the peptidergic neuroendocrine, endocrine, and nervous systems of vertebrates with certain specific molecular diversity.

Original languageEnglish
Pages (from-to)2416-2427
Number of pages12
JournalEndocrinology
Volume152
Issue number6
DOIs
Publication statusPublished - 2011 Jun

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Ciona intestinalis
Vertebrates
Central Nervous System
Peptides
Urochordata
Neuropeptides
Neurotensin
Peptide Hormones
Invertebrates
Nonvertebrate Chordata
Galanin-Like Peptide
Galanin
Neurosecretory Systems
Endocrine System
Sea Urchins

ASJC Scopus subject areas

  • Endocrinology

Cite this

Peptidomic analysis of the central nervous system of the protochordate, Ciona intestinalis : Homologs and prototypes of vertebrate peptides and novel peptides. / Kawada, Tsuyoshi; Ogasawara, Michio; Sekiguchi, Toshio; Aoyama, Masato; Hotta, Kohji; Oka, Kotaro; Satake, Honoo.

In: Endocrinology, Vol. 152, No. 6, 06.2011, p. 2416-2427.

Research output: Contribution to journalArticle

Kawada, Tsuyoshi ; Ogasawara, Michio ; Sekiguchi, Toshio ; Aoyama, Masato ; Hotta, Kohji ; Oka, Kotaro ; Satake, Honoo. / Peptidomic analysis of the central nervous system of the protochordate, Ciona intestinalis : Homologs and prototypes of vertebrate peptides and novel peptides. In: Endocrinology. 2011 ; Vol. 152, No. 6. pp. 2416-2427.
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