Perforin and Granzyme B of Cytotoxic T Lymphocyte Mediate Apoptosis Irrespective of Helicobacter pylori Infection: Possible Act as a Trigger of Peptic Ulcer Formation

Tadashi Ohara, Tetsuo Morishita, Hidekazu Suzuki, Tatsuhiro Masaoka, Hiromasa Ishii

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background/Aims: The perforin/granzyme and Fas/Fas ligand pathways are two known major pathways of cytotoxic T lymphocyte-mediated apoptosis. We designed a clinical study to examine whether cytotoxic T lymphocyte-mediated apoptosis associated with peptic ulcer formation may occur via either or both of these two pathways. Methodology: Mucosal biopsy specimens were obtained endoscopically from the marginal zone of active stage gastric and duodenal ulcers in patients with or without Helicobacter pylori infection. RT-PCR, immunoblotting and immunohistochemistry were used to study the samples for the expression of apoptotic cells, perforin, granzyme B, Fas, Fas ligand and caspase 3. Results: Apoptotic cells (Tunnel positive cells) appeared in the marginal zone of gastric and duodenal ulcers with and without H. pylori infection. Perforin/granzyme B and caspase 3 were expressed consistently, however Fas ligand was not. Furthermore, the immunohistochemical findings demonstrated apoptotic changes of target cells caused by perforin/granzyme B. Conclusions: These results suggest that the main pathway of cytotoxic T lymphocyte-mediated apoptosis in peptic ulcer formation is the perforin/ granzyme pathway irrespective of H. pylori infection.

Original languageEnglish
Pages (from-to)1774-1779
Number of pages6
JournalHepato-gastroenterology
Volume50
Issue number54
Publication statusPublished - 2003 Nov 1

Keywords

  • Apoptosis
  • CTL
  • Granzyme-B
  • Peptic ulcer
  • Perforin
  • Trigger of ulcer formation

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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