Pericellular versican regulates the fibroblast-myofibroblast transition: A role for ADAMTS5 protease-mediated proteolysis

Noriko Aramaki, David A. Carrino, Mark E. Lauer, Amit Vasanji, James D. Wylie, Courtney M. Nelson, Suneel S. Apte

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The cell and its glycosaminoglycan-rich pericellular matrix (PCM) comprise a functional unit. Because modification of PCM influences cell behavior, we investigated molecular mechanisms that regulate PCM volume and composition. In fibroblasts and other cells, aggregates of hyaluronan and versican are found in the PCM. Dermal fibroblasts from Adamts5 -/- mice, which lack a versican-degrading protease, ADAMTS5, had reduced versican proteolysis, increased PCM, altered cell shape, enhanced α-smooth muscle actin (SMA) expression and increased contractility within three-dimensional collagen gels. The myofibroblast-like phenotype was associated with activation of TGFβ signaling. We tested the hypothesis that fibroblast-myofibroblast transition in Adamts5 -/- cells resulted from versican accumulation in PCM. First, we noted that versican overexpression in human dermal fibroblasts led to increased SMA expression, enhanced contractility, and increased Smad2 phosphorylation. In contrast, dermal fibroblasts from Vcan haploinsufficient (Vcan hdf/+) mice had reduced contractility relative to wild type fibroblasts. Using a genetic approach to directly test if myofibroblast transition in Adamts5 -/- cells resulted from increased PCM versican content, we generated Adamts5 -/-;Vcan hdf/+ mice and isolated their dermal fibroblasts for comparison with dermal fibroblasts from Adamts5 -/-mice. In Adamts5 -/- fibroblasts, Vcan haploinsufficiency or exogenous ADAMTS5 restored normal fibroblast contractility. These findings demonstrate that altering PCM versican content through proteolytic activity of ADAMTS5 profoundly influenced the dermal fibroblast phenotype and may regulate a phenotypic continuum between the fibroblast and its alter ego, the myofibroblast. We propose that a physiological function of ADAMTS5 in dermal fibroblasts is to maintain optimal versican content and PCM volume by continually trimming versican in hyaluronan-versican aggregates.

Original languageEnglish
Pages (from-to)34298-34310
Number of pages13
JournalJournal of Biological Chemistry
Volume286
Issue number39
DOIs
Publication statusPublished - 2011 Oct 30
Externally publishedYes

Fingerprint

Versicans
Proteolysis
Myofibroblasts
Fibroblasts
Peptide Hydrolases
Skin
Hyaluronic Acid
ADAMTS5 Protein
Smooth Muscle
Muscle
Actins
Phenotype
Haploinsufficiency
Phosphorylation
Ego
Trimming
Cell Shape
Glycosaminoglycans

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Pericellular versican regulates the fibroblast-myofibroblast transition : A role for ADAMTS5 protease-mediated proteolysis. / Aramaki, Noriko; Carrino, David A.; Lauer, Mark E.; Vasanji, Amit; Wylie, James D.; Nelson, Courtney M.; Apte, Suneel S.

In: Journal of Biological Chemistry, Vol. 286, No. 39, 30.10.2011, p. 34298-34310.

Research output: Contribution to journalArticle

Aramaki, Noriko ; Carrino, David A. ; Lauer, Mark E. ; Vasanji, Amit ; Wylie, James D. ; Nelson, Courtney M. ; Apte, Suneel S. / Pericellular versican regulates the fibroblast-myofibroblast transition : A role for ADAMTS5 protease-mediated proteolysis. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 39. pp. 34298-34310.
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