TY - JOUR
T1 - Perioperative serum and urine metabolome analyses in patients with hepatocellular carcinoma undergoing partial hepatectomy
AU - Kajiura, Daisuke
AU - Yamanaka-Okumura, Hisami
AU - Hirayama, Akiyoshi
AU - Tatano, Hiroshi
AU - Endo, Keiko
AU - Honma, Mayu
AU - Igarashi, Kaori
AU - Shoji, Futaba
AU - Ikeda, Satsuki
AU - Yamaguchi, Nozomi
AU - Katayama, Takafumi
AU - Morine, Yuji
AU - Imura, Satoru
AU - Utsunomiya, Tohru
AU - Soga, Tomoyoshi
AU - Tomita, Masaru
AU - Shimada, Mitsuo
N1 - Funding Information:
This work was supported by research fund by Yamagata Prefecture and Tsuruoka City and Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (Research theme number: 16 K12738). DK, HY-O, and AH contributed equally to this work. DK and HY-O wrote the manuscript. HY-O contributed to the study design. HY-O and TU collected the samples. DK, HT, and TK analyzed the data. AH, KE, MH, KI, FS, SI, NY, and TS performed mass spectrometry and amino acid concentration analyses. AH, YM, SI, TS, MT, and MS provided helpful comments about the study. All authors read and approved the final manuscript. The authors have no conflicts of interest to declare.
Funding Information:
This study was supported by the Support Center for The Special Mission Center for Metabolome Analysis, School of Medical Nutrition, Faculty of Medicine of Tokushima University and the Support Center for Advanced Medical Sciences, Institute of Biomedical Sciences, and Tokushima University Graduate School provided materials
Publisher Copyright:
© 2018 Elsevier Ltd
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/2
Y1 - 2019/2
N2 - Objectives: Perioperative nutritional management is essential for early recovery after liver surgery. The aim of this study was to assess changes in amino acid levels in serum and urine after hepatectomy. Methods: Serum samples were collected from 16 patients with hepatocellular carcinoma before and 1, 3, and 14 d after hepatectomy (S0, S1, S3, and S14, respectively). Spot urine samples were collected before and 3 d after the hepatectomy (U0 and U3). Metabolites in the serum and urine were analyzed. Results: Compared with S0, insulin levels significantly increased in the S1 and S3 samples. Valine levels significantly decreased in S1 and S14, and leucine levels significantly decreased in S14. Phenylalanine levels significantly increased in S1 and S3, and tyrosine levels significantly increased in S1. The Fischer ratio (branched-chain/aromatic amino acids) significantly decreased in S1 and S3. In multiple regression analysis, changes in serum taurine levels were related to the white blood cell count in S1 and S3, and inversely related to alanine aminotransferase levels in S14. Changes in serum glutamine levels were negatively related to C-reactive protein levels in S3. Serum glutamine levels decreased in S3 and S14, and tended to increase in U3, suggesting a deficiency of glutamate resulting from the invasive surgical procedure. Conclusions: These findings highlight the usefulness of metabolome analysis for characterizing perioperative patterns after liver resection. The observed amino acid pattern, including the reduction in Fischer ratio, underscores the need for specialized nutritional support.
AB - Objectives: Perioperative nutritional management is essential for early recovery after liver surgery. The aim of this study was to assess changes in amino acid levels in serum and urine after hepatectomy. Methods: Serum samples were collected from 16 patients with hepatocellular carcinoma before and 1, 3, and 14 d after hepatectomy (S0, S1, S3, and S14, respectively). Spot urine samples were collected before and 3 d after the hepatectomy (U0 and U3). Metabolites in the serum and urine were analyzed. Results: Compared with S0, insulin levels significantly increased in the S1 and S3 samples. Valine levels significantly decreased in S1 and S14, and leucine levels significantly decreased in S14. Phenylalanine levels significantly increased in S1 and S3, and tyrosine levels significantly increased in S1. The Fischer ratio (branched-chain/aromatic amino acids) significantly decreased in S1 and S3. In multiple regression analysis, changes in serum taurine levels were related to the white blood cell count in S1 and S3, and inversely related to alanine aminotransferase levels in S14. Changes in serum glutamine levels were negatively related to C-reactive protein levels in S3. Serum glutamine levels decreased in S3 and S14, and tended to increase in U3, suggesting a deficiency of glutamate resulting from the invasive surgical procedure. Conclusions: These findings highlight the usefulness of metabolome analysis for characterizing perioperative patterns after liver resection. The observed amino acid pattern, including the reduction in Fischer ratio, underscores the need for specialized nutritional support.
KW - 3-Methylhistidine
KW - Branched-chain amino acids
KW - Hepatectomy
KW - Hepatocellular carcinoma
KW - Metabolomics
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U2 - 10.1016/j.nut.2018.06.002
DO - 10.1016/j.nut.2018.06.002
M3 - Article
C2 - 30391689
AN - SCOPUS:85055910165
VL - 58
SP - 110
EP - 119
JO - Nutrition
JF - Nutrition
SN - 0899-9007
ER -