Peripheral blood stem cell mobilization and apheresis: Analysis of adverse events in 94 normal donors

M. Murata, M. Harada, S. Kato, S. Takahashi, H. Ogawa, S. Okamoto, S. Tsuchiya, H. Sakamaki, Y. Akiyama, Y. Kodera

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74 Citations (Scopus)

Abstract

Adverse events were analyzed in 94 normal donors who underwent PBSC harvest with G-CSF. The median dose of G-CSF was 9.7 μg/kg/day (range, 2.0-16.7), and the duration of administration was 4-6 days. Frequent symptoms were bone pain (71%), general fatigue (33%), headache (28%), insomnia (14%), anorexia (11%), nausea and/or vomiting (11%). One donor (1%) developed grade 3 toxicity bone pain (WHO criteria). WBC counts and ANC increased during G-CSF administration. After leukapheresis, three donors (3%) developed grade 3 toxicity neutropenia. Platelet counts decreased after leukapheresis. Three donors (3%) developed grade 3 thrombocytopenia. The means of both ALP and LDH increased approximately 1.9-fold compared with pretreatment levels. In one pediatric donor (1%), ALP was elevated to the grade 3 toxicity level. From multivariate analysis, the incidence of bone pain increased when GCSF was given at a dose of 8.8 μg/kg/day or more, headaches were frequent in donors younger than 35 years, and the incidence of nausea and/or vomiting was high in female donors. The peak levels of WBC counts and ANC and post-treatment level of LDH increased in correspondence with the escalation of G-CSF dose. All adverse events normalized on follow-up evaluation. In conclusion, although PBSC harvest for normal donors is acceptable, care must be taken for all donors in terms of their sex and age as well as the G-CSF dose. We recommend less than 8.8 μg/kg/day as the G-CSF dose for PBSC mobilization in normal donors.

Original languageEnglish
Pages (from-to)1065-1071
Number of pages7
JournalBone Marrow Transplantation
Volume24
Issue number10
DOIs
Publication statusPublished - 1999 Jan 1

Keywords

  • Allogeneic transplantation
  • Apheresis
  • Donor
  • Granulocyte colony-stimulating factor
  • Mobilization
  • Peripheral blood stem cells

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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