Peripheral regulation of graft-versus-host alloreactivity in mixed chimeric miniature swine

Shaun M. Kunisaki, Gary W. Haller, Yasushi Fuchimoto, Christene A. Huang, David H. Sachs, G. Tenderich

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background. Despite the presence of circulating donor-derived T cells during the induction of mixed chimerism across MHC barriers in miniature swine, severe graft-versus-host disease was avoided in the majority of animals. In this study, we investigated the possible roles of recipient and donor lymphoid populations in the regulation of donor-anti-recipient alloreactivity. Methods. Mixed chimerism across a full MHC-mismatch barrier was established in miniature swine using a high-dose allogeneic peripheral blood stem cell protocol. Peripheral blood mononuclear cells from mixed chimeric swine were co-cultured with naïve donor-matched responders and naive recipient-matched stimulators in mixed lymphocyte reactions. Results. Peripheral blood mononuclear cells from mixed chimeras inhibited donor-anti-recipient proliferation. This suppression was radioresistant to 25 Gy. Suppression of donor-anti-recipient alloreactivity was not observed in mixed lymphocyte co-cultures when donor-derived cells were added in the absence of recipient-derived cells. Conclusions. These results suggest an association between the presence of an active and relatively radioresistant cell population, demonstrable in vitro, and the regulation of graft-versus-host disease across MHC barriers in mixed chimeric miniature swine.

Original languageEnglish
Pages (from-to)523-526
Number of pages4
JournalTransplantation
Volume72
Issue number3
DOIs
Publication statusPublished - 2001 Aug 15

ASJC Scopus subject areas

  • Transplantation

Fingerprint Dive into the research topics of 'Peripheral regulation of graft-versus-host alloreactivity in mixed chimeric miniature swine'. Together they form a unique fingerprint.

  • Cite this

    Kunisaki, S. M., Haller, G. W., Fuchimoto, Y., Huang, C. A., Sachs, D. H., & Tenderich, G. (2001). Peripheral regulation of graft-versus-host alloreactivity in mixed chimeric miniature swine. Transplantation, 72(3), 523-526. https://doi.org/10.1097/00007890-200108150-00027