TY - JOUR
T1 - Persistent Overexpression of Phosphoglycerate Mutase, a Glycolytic Enzyme, Modifies Energy Metabolism and Reduces Stress Resistance of Heart in Mice
AU - Okuda, Junji
AU - Niizuma, Shinnichiro
AU - Shioi, Tetsuo
AU - Kato, Takao
AU - Inuzuka, Yasutaka
AU - Kawashima, Tsuneaki
AU - Tamaki, Yodo
AU - Kawamoto, Akira
AU - Tanada, Yohei
AU - Iwanaga, Yoshitaka
AU - Narazaki, Michiko
AU - Matsuda, Tetsuya
AU - Adachi, Souichi
AU - Soga, Tomoyoshi
AU - Takemura, Genzou
AU - Kondoh, Hiroshi
AU - Kita, Toru
AU - Kimura, Takeshi
PY - 2013/8/12
Y1 - 2013/8/12
N2 - Background:Heart failure is associated with changes in cardiac energy metabolism. Glucose metabolism in particular is thought to be important in the pathogenesis of heart failure. We examined the effects of persistent overexpression of phosphoglycerate mutase 2 (Pgam2), a glycolytic enzyme, on cardiac energy metabolism and function.Methods and Results:Transgenic mice constitutively overexpressing Pgam2 in a heart-specific manner were generated, and cardiac energy metabolism and function were analyzed. Cardiac function at rest was normal. The uptake of analogs of glucose or fatty acids and the phosphocreatine/βATP ratio at rest were normal. A comprehensive metabolomic analysis revealed an increase in the levels of a few metabolites immediately upstream and downstream of Pgam2 in the glycolytic pathway, whereas the levels of metabolites in the initial few steps of glycolysis and lactate remained unchanged. The levels of metabolites in the tricarboxylic acid (TCA) cycle were altered. The capacity for respiration by isolated mitochondria in vitro was decreased, and that for the generation of reactive oxygen species (ROS) in vitro was increased. Impaired cardiac function was observed in response to dobutamine. Mice developed systolic dysfunction upon pressure overload.Conclusions:Constitutive overexpression of Pgam2 modified energy metabolism and reduced stress resistance of heart in mice.
AB - Background:Heart failure is associated with changes in cardiac energy metabolism. Glucose metabolism in particular is thought to be important in the pathogenesis of heart failure. We examined the effects of persistent overexpression of phosphoglycerate mutase 2 (Pgam2), a glycolytic enzyme, on cardiac energy metabolism and function.Methods and Results:Transgenic mice constitutively overexpressing Pgam2 in a heart-specific manner were generated, and cardiac energy metabolism and function were analyzed. Cardiac function at rest was normal. The uptake of analogs of glucose or fatty acids and the phosphocreatine/βATP ratio at rest were normal. A comprehensive metabolomic analysis revealed an increase in the levels of a few metabolites immediately upstream and downstream of Pgam2 in the glycolytic pathway, whereas the levels of metabolites in the initial few steps of glycolysis and lactate remained unchanged. The levels of metabolites in the tricarboxylic acid (TCA) cycle were altered. The capacity for respiration by isolated mitochondria in vitro was decreased, and that for the generation of reactive oxygen species (ROS) in vitro was increased. Impaired cardiac function was observed in response to dobutamine. Mice developed systolic dysfunction upon pressure overload.Conclusions:Constitutive overexpression of Pgam2 modified energy metabolism and reduced stress resistance of heart in mice.
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U2 - 10.1371/journal.pone.0072173
DO - 10.1371/journal.pone.0072173
M3 - Article
C2 - 23951293
AN - SCOPUS:84881527898
VL - 8
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 8
M1 - e72173
ER -