PFG acted as an inducer of premature senescence in TIG-1 normal diploid fibroblast and an inhibitor of mitosis in the HeLa cells

Ying Huang, Osamu Ohno, Kenji Miyamoto

Research output: Contribution to journalArticle

Abstract

Our previous work has reported an anti-proliferative compound from moutan cortex, paeoniflorigenone which can induce cancer-selective apoptosis. However, its anti-proliferative mechanism is still unknown. According to morphology changes (hypertrophy and flattening), we hypothesized that PFG can induce senescence or inhibit cell mitosis. Here we show that PFG can induce cellular senescence, evidenced by the expression of senescence-associated β-galactosidase, G0/G1 cell cycle arrest and permanent loss of proliferative ability, in normal TIG-1 diploid fibroblast but not cancerous HeLa cells. In cancerous HeLa cells, PFG inhibited proliferation by inducing S and G2/M cell cycle arrest and mitosis inhibition. DNA damage response was activated by PFG, interestingly the reactive oxygen species level was suppressed instead of escalated. To sum up, we report 3 new roles of PFG as, 1. inducer of premature senescence in normal TIG-1 cells, 2. inhibitor of mitosis in cancerous HeLa cells, 3. ROS scavenger. Abbreviations: PFG: Paeoniflorigenone; ROS: reactive oxygen species; ATM: ataxia telangiectasia mutated; t-BHP: tert-butyl hydroperoxide; SA-β-gal: senescence-associatedβ-galactosidase; DNA-PKcs: DNA-dependent protein kinase; γ-H2AX: H2AX phosphoryla-tion at Ser-139.

Original languageEnglish
Pages (from-to)986-995
Number of pages10
JournalBioscience, Biotechnology, and Biochemistry
Volume83
Issue number6
DOIs
Publication statusPublished - 2019 Jun 1

Fingerprint

Galactosidases
Fibroblasts
Diploidy
HeLa Cells
Mitosis
Reactive Oxygen Species
Cells
DNA-Activated Protein Kinase
tert-Butylhydroperoxide
DNA
Automatic teller machines
G2 Phase Cell Cycle Checkpoints
Apoptosis
G1 Phase Cell Cycle Checkpoints
Ataxia Telangiectasia
Cell Aging
Hypertrophy
DNA Damage
paeoniflorigenone
Neoplasms

Keywords

  • anti-proliferative
  • cell cycle arrest
  • Paeoniflorigenone
  • reactive oxygen species
  • senescence

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

PFG acted as an inducer of premature senescence in TIG-1 normal diploid fibroblast and an inhibitor of mitosis in the HeLa cells. / Huang, Ying; Ohno, Osamu; Miyamoto, Kenji.

In: Bioscience, Biotechnology, and Biochemistry, Vol. 83, No. 6, 01.06.2019, p. 986-995.

Research output: Contribution to journalArticle

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