TY - JOUR
T1 - pH-taxis drives aerobic bacteria in duodenum to migrate into the pancreas with tumors
AU - Shirai, Hiroaki
AU - Ito, Cocoro
AU - Tsukada, Kosuke
N1 - Funding Information:
This research is financially supported by the Keio Gijuku Fukuzawa Memorial Fund for the Advancement of Education and Research. We acknowledge the provision of data for pH taxis by Prof. Mertin Sitti of the Max Planck Institute. We greatly acknowledge discussions with Dr. Masayasu Horibe and Dr. Eisuke Iwasaki of School of Medicine, Department of Internal Medicine (Gastroenterology and Hepatology) of Keio University. We acknowledge the Keio University manufacturing center for creating the metal mold for the microfluidic device.
Funding Information:
This research is financially supported by the Keio Gijuku Fukuzawa Memorial Fund for the Advancement of Education and Research. We acknowledge the provision of data for pH taxis by Prof. Mertin Sitti of the Max Planck Institute. We greatly acknowledge discussions with Dr. Masayasu Horibe and Dr. Eisuke Iwasaki of School of Medicine, Department of Internal Medicine (Gastroenterology and Hepatology) of Keio University. We acknowledge the Keio University manufacturing center for creating the metal mold for the microfluidic device.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - As oral or intestinal bacteria have been found in pancreatic cystic fluid and tumors, understanding bacterial migration from the duodenum into the pancreas via hepato-pancreatic duct is critical. Mathematical models of migration of aerobic bacteria from the duodenum to the pancreas with tumors were developed. Additionally, the bacterial distributions under the pH gradient and those under flow were measured in double-layer flow based microfluidic device and T-shaped cylinders. Migration of aerobic bacteria from the duodenum into pancreas is counteracted by bile and pancreatic juice flow but facilitated by pH-taxis from acidic duodenum fluid toward more favorable slightly alkaline pH in pancreatic juice. Additionally, the reduced flow velocity in cancer patients, due to compressed pancreatic duct by solid tumor, facilitates migration. Moreover, measured distribution of GFP E. coli under the pH gradient in a microfluidic device validated pH-tactic behaviors. Furthermore, Pseudomonas fluorescens in hydrochloride solution, but not in bicarbonate solution, migrated upstream against bicarbonate flow of > 20 μm/s, with an advancement at approximately 50 μm/s.
AB - As oral or intestinal bacteria have been found in pancreatic cystic fluid and tumors, understanding bacterial migration from the duodenum into the pancreas via hepato-pancreatic duct is critical. Mathematical models of migration of aerobic bacteria from the duodenum to the pancreas with tumors were developed. Additionally, the bacterial distributions under the pH gradient and those under flow were measured in double-layer flow based microfluidic device and T-shaped cylinders. Migration of aerobic bacteria from the duodenum into pancreas is counteracted by bile and pancreatic juice flow but facilitated by pH-taxis from acidic duodenum fluid toward more favorable slightly alkaline pH in pancreatic juice. Additionally, the reduced flow velocity in cancer patients, due to compressed pancreatic duct by solid tumor, facilitates migration. Moreover, measured distribution of GFP E. coli under the pH gradient in a microfluidic device validated pH-tactic behaviors. Furthermore, Pseudomonas fluorescens in hydrochloride solution, but not in bicarbonate solution, migrated upstream against bicarbonate flow of > 20 μm/s, with an advancement at approximately 50 μm/s.
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U2 - 10.1038/s41598-022-05554-8
DO - 10.1038/s41598-022-05554-8
M3 - Article
C2 - 35110595
AN - SCOPUS:85123973005
SN - 2045-2322
VL - 12
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 1783
ER -
