Pharmacological differentiation of presynaptic M1 muscarinic receptors modulating acetylcholine release from postsynaptic muscarinic receptors in guinea-pig ileum

Koichiro Kawashima, Kazuko Fujimoto, Takeshi Suzuki, Hisayo Oohata

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

1. 1. Effects of three muscarinic antagonists on electrically evoked ACh release and contractile response were investigated in longitudinal muscle strips of guinea-pig ileum suspended in an organ-bath and superfused with Krebs solution. ACh release was determined by a specific radioimmunoassay. 2. 2. Telenzepine, a selective M1 muscarinic antagonist, increased the ACh release at a concentration of 100-fold less than that inhibiting the contractile response (10 vs 1000 nM). 3. 3. AF-DX 116, a cardioselective M2 muscarinic antagonist, inhibited the contractile response at 10 μM, but did not affect the ACh release at this concentration. 4. 4. (-)N-Methylscopolamine (NMS) did not affect the ACh release, but inhibited the contractile response at all concentrations tested (1-1000 nM), indicating (-)NMS can be used as an ileal specific postsynaptic muscarinic antagonist. 5. 5. These data demonstrate that presynaptic muscarinic receptors modulating ACh release are distinct from postsynaptic ones involved in the contractile response and can be classified as M1 subtype.

Original languageEnglish
Pages (from-to)17-21
Number of pages5
JournalGeneral Pharmacology
Volume21
Issue number1
DOIs
Publication statusPublished - 1990

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Muscarinic M1 Receptors
Muscarinic Antagonists
Muscarinic Receptors
Ileum
Guinea Pigs
N-Methylscopolamine
Pharmacology
Presynaptic Receptors
Baths
Radioimmunoassay
Muscles

ASJC Scopus subject areas

  • Pharmacology

Cite this

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title = "Pharmacological differentiation of presynaptic M1 muscarinic receptors modulating acetylcholine release from postsynaptic muscarinic receptors in guinea-pig ileum",
abstract = "1. 1. Effects of three muscarinic antagonists on electrically evoked ACh release and contractile response were investigated in longitudinal muscle strips of guinea-pig ileum suspended in an organ-bath and superfused with Krebs solution. ACh release was determined by a specific radioimmunoassay. 2. 2. Telenzepine, a selective M1 muscarinic antagonist, increased the ACh release at a concentration of 100-fold less than that inhibiting the contractile response (10 vs 1000 nM). 3. 3. AF-DX 116, a cardioselective M2 muscarinic antagonist, inhibited the contractile response at 10 μM, but did not affect the ACh release at this concentration. 4. 4. (-)N-Methylscopolamine (NMS) did not affect the ACh release, but inhibited the contractile response at all concentrations tested (1-1000 nM), indicating (-)NMS can be used as an ileal specific postsynaptic muscarinic antagonist. 5. 5. These data demonstrate that presynaptic muscarinic receptors modulating ACh release are distinct from postsynaptic ones involved in the contractile response and can be classified as M1 subtype.",
author = "Koichiro Kawashima and Kazuko Fujimoto and Takeshi Suzuki and Hisayo Oohata",
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AU - Fujimoto, Kazuko

AU - Suzuki, Takeshi

AU - Oohata, Hisayo

PY - 1990

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N2 - 1. 1. Effects of three muscarinic antagonists on electrically evoked ACh release and contractile response were investigated in longitudinal muscle strips of guinea-pig ileum suspended in an organ-bath and superfused with Krebs solution. ACh release was determined by a specific radioimmunoassay. 2. 2. Telenzepine, a selective M1 muscarinic antagonist, increased the ACh release at a concentration of 100-fold less than that inhibiting the contractile response (10 vs 1000 nM). 3. 3. AF-DX 116, a cardioselective M2 muscarinic antagonist, inhibited the contractile response at 10 μM, but did not affect the ACh release at this concentration. 4. 4. (-)N-Methylscopolamine (NMS) did not affect the ACh release, but inhibited the contractile response at all concentrations tested (1-1000 nM), indicating (-)NMS can be used as an ileal specific postsynaptic muscarinic antagonist. 5. 5. These data demonstrate that presynaptic muscarinic receptors modulating ACh release are distinct from postsynaptic ones involved in the contractile response and can be classified as M1 subtype.

AB - 1. 1. Effects of three muscarinic antagonists on electrically evoked ACh release and contractile response were investigated in longitudinal muscle strips of guinea-pig ileum suspended in an organ-bath and superfused with Krebs solution. ACh release was determined by a specific radioimmunoassay. 2. 2. Telenzepine, a selective M1 muscarinic antagonist, increased the ACh release at a concentration of 100-fold less than that inhibiting the contractile response (10 vs 1000 nM). 3. 3. AF-DX 116, a cardioselective M2 muscarinic antagonist, inhibited the contractile response at 10 μM, but did not affect the ACh release at this concentration. 4. 4. (-)N-Methylscopolamine (NMS) did not affect the ACh release, but inhibited the contractile response at all concentrations tested (1-1000 nM), indicating (-)NMS can be used as an ileal specific postsynaptic muscarinic antagonist. 5. 5. These data demonstrate that presynaptic muscarinic receptors modulating ACh release are distinct from postsynaptic ones involved in the contractile response and can be classified as M1 subtype.

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