Phase II enzyme induction by a carotenoid, lutein, in a PC12D neuronal cell line

Seiji Miyake, Saori Kobayashi, Kazuo Tsubota, Yoko Ozawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The mechanism by which lutein, a carotenoid, acts as an antioxidant in retinal cells is still not fully understood. Here, lutein treatment of a neuronal cell line (PC12D) immediately resulted in reduced intracellular ROS levels, implying that it has a direct role in ROS scavenging. Significantly, lutein treatment also induced phase II antioxidative enzyme expression, probably via a nuclear factor-like 2 (Nrf2) independent pathway. This latter mechanism could explain why lutein acts diversely to protect against oxidative/cytotoxic stress, and why it is physiologically involved in the human neural tissue, such as the retina.

Original languageEnglish
Pages (from-to)535-540
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume446
Issue number2
DOIs
Publication statusPublished - 2014 Apr 4

Fingerprint

Lutein
Enzyme Induction
Carotenoids
Cells
Cell Line
Enzymes
Oxidative stress
Scavenging
Retina
Oxidative Stress
Antioxidants
Tissue

Keywords

  • Antioxidant enzymes
  • Lutein
  • Nrf2
  • PC12D
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology
  • Biochemistry

Cite this

Phase II enzyme induction by a carotenoid, lutein, in a PC12D neuronal cell line. / Miyake, Seiji; Kobayashi, Saori; Tsubota, Kazuo; Ozawa, Yoko.

In: Biochemical and Biophysical Research Communications, Vol. 446, No. 2, 04.04.2014, p. 535-540.

Research output: Contribution to journalArticle

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