Phase II enzyme induction by a carotenoid, lutein, in a PC12D neuronal cell line

Seiji Miyake; Saori Kobayashi; Kazuo Tsubota; Yoko Ozawa

doi:10.1016/j.bbrc.2014.02.135

Phase II enzyme induction by a carotenoid, lutein, in a PC12D neuronal cell line

Seiji Miyake, Saori Kobayashi, Kazuo Tsubota, Yoko Ozawa

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The mechanism by which lutein, a carotenoid, acts as an antioxidant in retinal cells is still not fully understood. Here, lutein treatment of a neuronal cell line (PC12D) immediately resulted in reduced intracellular ROS levels, implying that it has a direct role in ROS scavenging. Significantly, lutein treatment also induced phase II antioxidative enzyme expression, probably via a nuclear factor-like 2 (Nrf2) independent pathway. This latter mechanism could explain why lutein acts diversely to protect against oxidative/cytotoxic stress, and why it is physiologically involved in the human neural tissue, such as the retina.

Original language	English
Pages (from-to)	535-540
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	446
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2014.02.135
Publication status	Published - 2014 Apr 4
Externally published	Yes

Keywords

Antioxidant enzymes
Lutein
Nrf2
PC12D
Reactive oxygen species

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2014.02.135

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title = "Phase II enzyme induction by a carotenoid, lutein, in a PC12D neuronal cell line",

abstract = "The mechanism by which lutein, a carotenoid, acts as an antioxidant in retinal cells is still not fully understood. Here, lutein treatment of a neuronal cell line (PC12D) immediately resulted in reduced intracellular ROS levels, implying that it has a direct role in ROS scavenging. Significantly, lutein treatment also induced phase II antioxidative enzyme expression, probably via a nuclear factor-like 2 (Nrf2) independent pathway. This latter mechanism could explain why lutein acts diversely to protect against oxidative/cytotoxic stress, and why it is physiologically involved in the human neural tissue, such as the retina.",

keywords = "Antioxidant enzymes, Lutein, Nrf2, PC12D, Reactive oxygen species",

author = "Seiji Miyake and Saori Kobayashi and Kazuo Tsubota and Yoko Ozawa",

note = "Funding Information: We thank Ms. Eriko Toda for technical assistance. This study was supported by a grant from Wakasa Seikatsu Co., Ltd., and partly by a grant-in-aid from JSPS KAKENHI to YO (24592647). Seiji Miyake and Saori Kobayashi were employed by Wakasa Seikatsu Co., Ltd. ",

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doi = "10.1016/j.bbrc.2014.02.135",

language = "English",

volume = "446",

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journal = "Biochemical and Biophysical Research Communications",

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AU - Miyake, Seiji

AU - Kobayashi, Saori

AU - Tsubota, Kazuo

AU - Ozawa, Yoko

N1 - Funding Information: We thank Ms. Eriko Toda for technical assistance. This study was supported by a grant from Wakasa Seikatsu Co., Ltd., and partly by a grant-in-aid from JSPS KAKENHI to YO (24592647). Seiji Miyake and Saori Kobayashi were employed by Wakasa Seikatsu Co., Ltd.

PY - 2014/4/4

Y1 - 2014/4/4

N2 - The mechanism by which lutein, a carotenoid, acts as an antioxidant in retinal cells is still not fully understood. Here, lutein treatment of a neuronal cell line (PC12D) immediately resulted in reduced intracellular ROS levels, implying that it has a direct role in ROS scavenging. Significantly, lutein treatment also induced phase II antioxidative enzyme expression, probably via a nuclear factor-like 2 (Nrf2) independent pathway. This latter mechanism could explain why lutein acts diversely to protect against oxidative/cytotoxic stress, and why it is physiologically involved in the human neural tissue, such as the retina.

AB - The mechanism by which lutein, a carotenoid, acts as an antioxidant in retinal cells is still not fully understood. Here, lutein treatment of a neuronal cell line (PC12D) immediately resulted in reduced intracellular ROS levels, implying that it has a direct role in ROS scavenging. Significantly, lutein treatment also induced phase II antioxidative enzyme expression, probably via a nuclear factor-like 2 (Nrf2) independent pathway. This latter mechanism could explain why lutein acts diversely to protect against oxidative/cytotoxic stress, and why it is physiologically involved in the human neural tissue, such as the retina.

KW - Antioxidant enzymes

KW - Lutein

KW - Nrf2

KW - PC12D

KW - Reactive oxygen species

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Phase II enzyme induction by a carotenoid, lutein, in a PC12D neuronal cell line

Abstract

Keywords

ASJC Scopus subject areas

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