TY - JOUR
T1 - Phemx, a novel mouse gene expressed in hematopoietic cells maps to the imprinted cluster on distal chromosome 7
AU - Nicholson, Rhonda H.
AU - Pantano, Serafino
AU - Eliason, James F.
AU - Galy, Anne
AU - Weiler, Sarah
AU - Kaplan, Joseph
AU - Hughes, Mark R.
AU - Ko, Minoru S.H.
N1 - Funding Information:
We thank Marija J. Grahovac, Tracy A. Threat, Harry Dawson, Audrey N. Kalehua, and the Flow Cytometry Core Facility of the Karmanos Cancer Institute for assistance, and Ben Chen for the generous gift of cultured murine macrophages. We also thank David Schlessinger and Dan Longo for discussion and for critically reading the manuscript. The work was partially supported by NIH Grant (HD32248) to M.S.H.K., and was also supported by the NIA/NIH Intramural Research Program.
PY - 2000/8/15
Y1 - 2000/8/15
N2 - Phemx (Pan hematopoietic expression) is a novel murine gene expressed in developmentally regulated sites of hematopoiesis from early in embryogenesis through adulthood. Phemx is expressed in hematopoietic progenitors and mature cells of the three main hematopoietic lineages. Conceptual translation of the murine Phemx cDNA predicts a 25-kDa polypeptide with four hydrophobic regions and several potential phosphorylation sites, suggestive of a transmembrane protein involved in cell signaling. The PHEMX protein is structurally similar to tetraspanin CD81 (TAPA-1), a transmembrane protein involved in leukocyte activation, adhesion, and proliferation. Phemx maps to the distal region of chromosome 7, a segment of the mouse genome that contains a cluster of genes that exhibit genomic imprinting. However, imprinting analysis of Phemx at the whole organ level shows that it is biallelically expressed, suggesting that mechanisms leading to monoallelic expression are not imposed at this locus. The human PHEMX ortholog is specifically expressed in hematopoietic organs and tissues and, in contrast to murine Phemx, undergoes alternative splicing. The unique mode and range of Phemx expression suggest that it plays a role in hematopoietic cell function. (C) 2000 Academic Press.
AB - Phemx (Pan hematopoietic expression) is a novel murine gene expressed in developmentally regulated sites of hematopoiesis from early in embryogenesis through adulthood. Phemx is expressed in hematopoietic progenitors and mature cells of the three main hematopoietic lineages. Conceptual translation of the murine Phemx cDNA predicts a 25-kDa polypeptide with four hydrophobic regions and several potential phosphorylation sites, suggestive of a transmembrane protein involved in cell signaling. The PHEMX protein is structurally similar to tetraspanin CD81 (TAPA-1), a transmembrane protein involved in leukocyte activation, adhesion, and proliferation. Phemx maps to the distal region of chromosome 7, a segment of the mouse genome that contains a cluster of genes that exhibit genomic imprinting. However, imprinting analysis of Phemx at the whole organ level shows that it is biallelically expressed, suggesting that mechanisms leading to monoallelic expression are not imposed at this locus. The human PHEMX ortholog is specifically expressed in hematopoietic organs and tissues and, in contrast to murine Phemx, undergoes alternative splicing. The unique mode and range of Phemx expression suggest that it plays a role in hematopoietic cell function. (C) 2000 Academic Press.
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U2 - 10.1006/geno.2000.6277
DO - 10.1006/geno.2000.6277
M3 - Article
C2 - 10950922
AN - SCOPUS:0034662761
VL - 68
SP - 13
EP - 21
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 1
ER -