Phenolic compounds from the bark of Oroxylum indicum activate the Ngn2 promoter

Rolly G. Fuentes, Midori A. Arai, Samir K. Sadhu, Firoj Ahmed, Masami Ishibashi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


A reporter gene assay that detects neurogenin 2 (Ngn2) promoter activity was utilized to identify compounds that induce neuronal differentiation. Ngn2 is a basic helix-loop-helix transcription factor that activates transcription of pro-neural genes. Using this assay system and an activity-guided approach, seven phenolic compounds were isolated from the methanol extract of Oroxylum indicum: 1 oroxylin A, 2 chrysin, 3 hispidulin, 4 baicalein, 5 apigenin, 6 baicalin, and 7 isoverbascoside. Compounds 1 and 2 induced an estimated 2.7-fold increase in Ngn2 promoter activity, whereas 3 increased the activity by 2.5-fold. Furthermore, 1 and 2 enhanced neuronal differentiation of C17.2 cells, which are multipotent stem cells. Graphical Abstract: [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)589-594
Number of pages6
JournalJournal of Natural Medicines
Issue number4
Publication statusPublished - 2015 Oct 11
Externally publishedYes


  • Flavonoids
  • Neurite
  • bHLH

ASJC Scopus subject areas

  • Molecular Medicine


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