Phenolic compounds from the bark of Oroxylum indicum activate the Ngn2 promoter

Rolly G. Fuentes, Midori A. Arai, Samir K. Sadhu, Firoj Ahmed, Masami Ishibashi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A reporter gene assay that detects neurogenin 2 (Ngn2) promoter activity was utilized to identify compounds that induce neuronal differentiation. Ngn2 is a basic helix-loop-helix transcription factor that activates transcription of pro-neural genes. Using this assay system and an activity-guided approach, seven phenolic compounds were isolated from the methanol extract of Oroxylum indicum: 1 oroxylin A, 2 chrysin, 3 hispidulin, 4 baicalein, 5 apigenin, 6 baicalin, and 7 isoverbascoside. Compounds 1 and 2 induced an estimated 2.7-fold increase in Ngn2 promoter activity, whereas 3 increased the activity by 2.5-fold. Furthermore, 1 and 2 enhanced neuronal differentiation of C17.2 cells, which are multipotent stem cells. Graphical Abstract: [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)589-594
Number of pages6
JournalJournal of Natural Medicines
Volume69
Issue number4
DOIs
Publication statusPublished - 2015 Oct 11
Externally publishedYes

Keywords

  • bHLH
  • Flavonoids
  • Neurite

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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