Phenotypes of dnaA mutants of Escherichia coli sensitive to phenothiazine derivatives

Tohru Mizushima, Tomoko Shinpuku, Hideki Katayama, Kazuhiro Kataoka, Lei Guo, Takeyoshi Miki, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

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Abstract

The activation of DnaA protein by cardiolipin is inhibited by fluphenazine in vitro. We therefore examined the sensitivity of temperature-sensitive dnaA mutants of Escherichia coli to fluphenazine and other phenothiazine derivatives. Among the eight dnaA mutants tested, dnaA5, dnaA46 dnaA602, and dnaA604, mutants with mutations in the putative ATP binding site of DnaA protein, showed higher sensitivities to phenothiazine derivatives than did the wild-type strain. The dnaA508 and dnaA167 mutants, which have mutations in the N-terminal region of DnaA protein, also showed higher sensitivities to phenothiazine derivatives. On the other hand, the dnaA204 and dnaA205 mutants, with lesions in the C-terminal region of the DnaA protein, showed the same sensitivity to phenothiazine derivatives as the wild-type strain. Complementation analysis with a plasmid containing the wild-type dnaA gene and phage P1-mediated transduction confirmed that dnaA mutations are responsible for these sensitivity phenotypes.

Original languageEnglish
Pages (from-to)212-215
Number of pages4
JournalMolecular and General Genetics
Volume252
Issue number1-2
DOIs
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Escherichia coli
Fluphenazine
Phenotype
Mutation
Proteins
Bacteriophage P1
Cardiolipins
Plasmids
Adenosine Triphosphate
Binding Sites
Temperature
phenothiazine
Genes
In Vitro Techniques

Keywords

  • DnaA protein
  • Escherichia coli
  • Phenothiazine derivatives

ASJC Scopus subject areas

  • Genetics

Cite this

Mizushima, T., Shinpuku, T., Katayama, H., Kataoka, K., Guo, L., Miki, T., & Sekimizu, K. (1996). Phenotypes of dnaA mutants of Escherichia coli sensitive to phenothiazine derivatives. Molecular and General Genetics, 252(1-2), 212-215. https://doi.org/10.1007/s004389670024

Phenotypes of dnaA mutants of Escherichia coli sensitive to phenothiazine derivatives. / Mizushima, Tohru; Shinpuku, Tomoko; Katayama, Hideki; Kataoka, Kazuhiro; Guo, Lei; Miki, Takeyoshi; Sekimizu, Kazuhisa.

In: Molecular and General Genetics, Vol. 252, No. 1-2, 1996, p. 212-215.

Research output: Contribution to journalArticle

Mizushima, T, Shinpuku, T, Katayama, H, Kataoka, K, Guo, L, Miki, T & Sekimizu, K 1996, 'Phenotypes of dnaA mutants of Escherichia coli sensitive to phenothiazine derivatives', Molecular and General Genetics, vol. 252, no. 1-2, pp. 212-215. https://doi.org/10.1007/s004389670024
Mizushima, Tohru ; Shinpuku, Tomoko ; Katayama, Hideki ; Kataoka, Kazuhiro ; Guo, Lei ; Miki, Takeyoshi ; Sekimizu, Kazuhisa. / Phenotypes of dnaA mutants of Escherichia coli sensitive to phenothiazine derivatives. In: Molecular and General Genetics. 1996 ; Vol. 252, No. 1-2. pp. 212-215.
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